Factors affecting high-frequency fungal protoplast fusion

L. Ferenczy, F. Kevei, M. Szegedi, A. Frankó, I. Rojik

Research output: Contribution to journalArticle

22 Citations (Scopus)


Factors influencing the fusion frequency of protoplasts have been examined with auxotrophic mutants of Aspergillus nidulans. The optimum conditions were a total of 5 to 15 million protoplasts per ml, and 25% polyethylene glycol (PEG) 4000 or 6000 as fusogenic agent in 10 to 100 m M CaCl2 solution.

Original languageEnglish
Pages (from-to)1156-1158
Number of pages3
Issue number9
Publication statusPublished - Sep 1 1976

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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