Factors affecting high-frequency fungal protoplast fusion

L. Ferenczy, F. Kevei, M. Szegedi, A. Frankó, I. Rojik

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Factors influencing the fusion frequency of protoplasts have been examined with auxotrophic mutants of Aspergillus nidulans. The optimum conditions were a total of 5 to 15 million protoplasts per ml, and 25% polyethylene glycol (PEG) 4000 or 6000 as fusogenic agent in 10 to 100 m M CaCl2 solution.

Original languageEnglish
Pages (from-to)1156-1158
Number of pages3
JournalExperientia
Volume32
Issue number9
DOIs
Publication statusPublished - Sep 1976

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Protoplasts
Aspergillus
Fusion reactions
Aspergillus nidulans
polyethylene glycol 4000

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Factors affecting high-frequency fungal protoplast fusion. / Ferenczy, L.; Kevei, F.; Szegedi, M.; Frankó, A.; Rojik, I.

In: Experientia, Vol. 32, No. 9, 09.1976, p. 1156-1158.

Research output: Contribution to journalArticle

Ferenczy, L. ; Kevei, F. ; Szegedi, M. ; Frankó, A. ; Rojik, I. / Factors affecting high-frequency fungal protoplast fusion. In: Experientia. 1976 ; Vol. 32, No. 9. pp. 1156-1158.
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