Factor XIII of blood coagulation as a nuclear crosslinking enzyme

R. Ádány, H. Bárdos, M. Antal, L. Módis, A. Sárváry, S. Szücs, I. Balogh

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Intracellular localization and distribution of Factor XIII subunit A (FXIIIA) was investigated in association with monocyte-macrophage differentiation in a long term culture of human monocytes by light- and electron microscopical as well as biochemical and immunobiochemical techniques. To allow the detection of FXIIIA in cells with well-preserved ultrustructure, immunosera against glutaraldehyde-derivatized recombinant FXIIIA were developed in rabbits, then characterized and used in this study. In the early phase of macrophage differentiation intranuclear accumulation of FXIIIA was detected as a transient phenomenon in cells of the 2nd day culture by optical sectioning with 0,7 μm steps in laser scanning confocal microscopy and immunoblotting technique. FXIIIA could be detected by immunoelectron microscopic postembedding staining over electrodense DNA-containing areas. Fluoresceinated monodansylcadaverine incorporation assay was used to demonstrate that FXIIIA is not only present in the nuclei, but also expresses its transglutaminase activity. Our finding of the nuclear accumulation of FXIIIA in differentiating human macrophages is also unique in that a blood clotting factor has, for the first time, been localized in nuclei and has been shown to be an intracellular crosslinking enzyme. The possible role of nuclear FXIIIA in association with cellular processes involving chromatin structure remodeling, such as cell death, cell differentiation or cellular proliferation requires further in-depth investigation.

Original languageEnglish
Pages (from-to)845-851
Number of pages7
JournalThrombosis and Haemostasis
Volume85
Issue number5
Publication statusPublished - 2001

Fingerprint

Factor XIII
Enzymes
Macrophages
Monocytes
Cell Proliferation
Transglutaminases
Chromatin Assembly and Disassembly
Blood Coagulation Factors
factor XIII subunit A
Glutaral
Blood Coagulation
Immunoblotting
Confocal Microscopy
Cell Differentiation
Cell Death
Electrons
Staining and Labeling
Rabbits
Light
DNA

Keywords

  • Cell-nucleus
  • Confocal microscopy
  • Factor XIII
  • Monocyte-macrophage differentiation
  • Transglutaminase

ASJC Scopus subject areas

  • Hematology

Cite this

Factor XIII of blood coagulation as a nuclear crosslinking enzyme. / Ádány, R.; Bárdos, H.; Antal, M.; Módis, L.; Sárváry, A.; Szücs, S.; Balogh, I.

In: Thrombosis and Haemostasis, Vol. 85, No. 5, 2001, p. 845-851.

Research output: Contribution to journalArticle

Ádány, R, Bárdos, H, Antal, M, Módis, L, Sárváry, A, Szücs, S & Balogh, I 2001, 'Factor XIII of blood coagulation as a nuclear crosslinking enzyme', Thrombosis and Haemostasis, vol. 85, no. 5, pp. 845-851.
Ádány, R. ; Bárdos, H. ; Antal, M. ; Módis, L. ; Sárváry, A. ; Szücs, S. ; Balogh, I. / Factor XIII of blood coagulation as a nuclear crosslinking enzyme. In: Thrombosis and Haemostasis. 2001 ; Vol. 85, No. 5. pp. 845-851.
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