Fabrication of optical multilayer for two-color phase plate in super-resolution microscope

Yoshinori Iketaki, Katsuichi Kitagawa, Kohjiro Hidaka, Naoki Kato, Akira Hirabayashi, N. Bokor

Research output: Contribution to journalArticle

Abstract

In super-resolution microscopy based on fluorescence depletion, the two-color phase plate (TPP) is an indispensable optical element, which can independently control the phase shifts for two beams of different color, i.e., the pump and erase beams. By controlling a phase shift of the erase beam through the TPP, the erase beam can be modulated into a doughnut shape, while the pump beam maintains the initial Gaussian shape. To obtain a reliable optical multiplayer (ML) for the TPP, we designed a ML with only two optical layers by performing numerical optimization. The measured phase shifts generated by the fabricated ML using interferometry correspond to the design values. The beam profiles in the focal plane are also consistent with theoretical results. Although the fabricated ML consists of only two optical layers, the ML can provide a suitable phase modulation function for the TPP in a practical super-resolution microscope.

Original languageEnglish
Article number073701
JournalReview of Scientific Instruments
Volume85
Issue number7
DOIs
Publication statusPublished - 2014

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Optical multilayers
Microscopes
microscopes
Color
color
Fabrication
fabrication
Phase shift
phase shift
Pumps
pumps
Phase modulation
Optical devices
Interferometry
Microscopic examination
phase modulation
Fluorescence
interferometry
depletion
microscopy

ASJC Scopus subject areas

  • Instrumentation

Cite this

Fabrication of optical multilayer for two-color phase plate in super-resolution microscope. / Iketaki, Yoshinori; Kitagawa, Katsuichi; Hidaka, Kohjiro; Kato, Naoki; Hirabayashi, Akira; Bokor, N.

In: Review of Scientific Instruments, Vol. 85, No. 7, 073701, 2014.

Research output: Contribution to journalArticle

Iketaki, Yoshinori ; Kitagawa, Katsuichi ; Hidaka, Kohjiro ; Kato, Naoki ; Hirabayashi, Akira ; Bokor, N. / Fabrication of optical multilayer for two-color phase plate in super-resolution microscope. In: Review of Scientific Instruments. 2014 ; Vol. 85, No. 7.
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