Extramitochondrial porin: Facts and hypotheses

György Báthori, Isabella Parolini, Ildikó Szabó, Francesco Tombola, Angela Messina, Marta Oliva, Massimo Sargiacomo, Vito De Pinto, Mario Zoratti

Research output: Contribution to journalReview article

50 Citations (Scopus)

Abstract

Mitochondrial porin, or VDAC, is a pore-forming protein abundant in the outer mitochondrial membrane. Several publications have reported extramitochondrial localizations as well, but the evidence was considered insufficient by many, and the presence of porin in nonmitochondrial cellular compartments has remained in doubt for a long time. We have now obtained new data indicating that the plasma membrane of hematopoietic cells contains porin, probably located mostly in caveolae or caveolae-like domains. Porin was purified from the plasma membrane of intact cells by a procedure utilizing the membrane-impermeable labeling reagent NH-SS-biotin and streptavidin affinity chromatography, and shown to have the same properties as mitochondrial porin. A channel with properties similar to that of isolated VDAC was observed by patch-clamping intact cells. This review discusses the evidence supporting extramitochondrial localization, the putative identification of the plasma membrane porin with the 'maxi' chloride channel, the hypothetical mechanisms of sorting porin to various cellular membrane structures, and its possible functions.

Original languageEnglish
Pages (from-to)79-89
Number of pages11
JournalJournal of Bioenergetics and Biomembranes
Volume32
Issue number1
DOIs
Publication statusPublished - May 16 2000

Keywords

  • Biotin
  • Caveolae
  • Cholesterol transport
  • Maxi chloride channel
  • Patch clamp
  • Plasmalemma
  • Porin
  • VDAC

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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  • Cite this

    Báthori, G., Parolini, I., Szabó, I., Tombola, F., Messina, A., Oliva, M., Sargiacomo, M., De Pinto, V., & Zoratti, M. (2000). Extramitochondrial porin: Facts and hypotheses. Journal of Bioenergetics and Biomembranes, 32(1), 79-89. https://doi.org/10.1023/A:1005516513313