Extramedullary hematopoiesis is dysregulated in histamine-free histidine decarboxylase knockout (HDC-/-) mice

Zsuzsanna Horváth, Éva Pállinger, Gyozo Horváth, Ivett Jelinek, Gizella Veszely, József Furész, András Falus, Edit I. Buzás

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective and design: In this study we investigated the role of histamine on the extramedullary hematopoiesis. Methods: Male histidine decarboxylase knockout (HDC-/-) mice and wild-type mice were used (n = 5/group). Groups of mice received sublethal total-body gamma irradiation at a single dose of 4 Gy. Spleen cells were studied at different time points post-irradiation by flow cytometry, colony forming unit (CFU) assay, and real-time PCR. For statistical analysis Student's t test, ANOVA, and Holm-Sidak post-hoc test were used. Results: By day 14 after irradiation, spleen cell counts increased almost eightfold in wild-type and not even fourfold in HDC-/- mice (P < 0.01). The proliferative capacity and interleukin-3 signaling of stem cells were impaired in HDC-/- mice. STAT5 mRNA expression was decreased in granulocyte-myeloid colonies by 72.9 ± 8.6% (P < 0.001), compared to the wild-type. Conclusions: The absence of histamine adversely affects splenic hematopoiesis via direct and indirect mechanisms.

Original languageEnglish
Pages (from-to)429-436
Number of pages8
JournalInflammation Research
Volume59
Issue number6
DOIs
Publication statusPublished - Jun 1 2010

Keywords

  • Hematopoiesis
  • Histamine
  • IL-3 signaling
  • Spleen
  • Transgenic mice

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

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