Extrachromosomal rDNA of Tetrahymena thermophila is not a perfect palindrome

Gyorgy B. Kiss, Ronald E. Pearlman

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

We have determined the restriction-endonuclease-cleavage map and the nucleotide sequence of the central 1.4 kb fragment of the macronuclear extrachromosomal rDNA of Tetrahymena thermophila. These data demonstrate that this molecule is not a perfect palindrome, having a 29 bp AT-rich non-palindromic sequence at its center. This observation is important in determining the mechanism by which a single chromosomally integrated rRNA gene in the micronucleus is rearranged and amplified during sexual development to yield multiple copies of extrachromosomal rDNA in the macronucleus.

Original languageEnglish
Pages (from-to)281-287
Number of pages7
JournalGene
Volume13
Issue number3
DOIs
Publication statusPublished - Apr 1981

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Keywords

  • Ribosomal RNA gene
  • excision
  • gene amplification
  • nucleotide sequence
  • origin of replication
  • restriction endonuclease maps

ASJC Scopus subject areas

  • Genetics

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