Extracellular vesicles mediate radiation-induced systemic bystander signals in the bone marrow and spleen

Tünde Szatmári, Dávid Kis, Eniko Noémi Bogdándi, Anett Benedek, Scott Bright, Deborah Bowler, Eszter Persa, Eniko Kis, Andrea Balogh, Lívia N. Naszályi, Munira Kadhim, G. Sáfrány, Katalin Lumniczky

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Radiation-induced bystander effects refer to the induction of biological changes in cells not directly hit by radiation implying that the number of cells affected by radiation is larger than the actual number of irradiated cells. Recent in vitro studies suggest the role of extracellular vesicles (EVs) in mediating radiation-induced bystander signals, but in vivo investigations are still lacking. Here, we report an in vivo study investigating the role of EVs in mediating radiation effects. C57BL/6 mice were total-body irradiated with X-rays (0.1,0.25, 2 Gy), and 24 h later, EVs were isolated from the bone marrow (BM) and were intravenously injected into unirradiated (so-called bystander) animals. EV-induced systemic effects were compared to radiation effects in the directly irradiated animals. Similar to direct radiation, EVs from irradiated mice induced complex DNA damage in EV-recipient animals, manifested in an increased level of chromosomal aberrations and the activation of the DNA damage response. However, while DNA damage after direct irradiation increased with the dose, EV-induced effects peaked at lower doses. A significantly reduced hematopoietic stem cell pool in the BM as well as CD4+ and CD8+ lymphocyte pool in the spleen was detected in mice injected with EVs isolated from animals irradiated with 2 Gy. These EV-induced alterations were comparable to changes present in the directly irradiated mice. The pool of TLR4-expressing dendritic cells was different in the directly irradiated mice, where it increased after 2 Gy and in the EV-recipient animals, where it strongly decreased in a dose-independent manner. A panel of eight differentially expressed microRNAs (miRNA) was identified in the EVs originating from both low- and high-dose-irradiated mice, with a predicted involvement in pathways related to DNA damage repair, hematopoietic, and immune system regulation, suggesting a direct involvement of these pathways in mediating radiation-induced systemic effects. In conclusion, we proved the role of EVs in transmitting certain radiation effects, identified miRNAs carried by EVs potentially responsible for these effects, and showed that the pattern of changes was often different in the directly irradiated and EV-recipient bystander mice, suggesting different mechanisms.

Original languageEnglish
Article number347
JournalFrontiers in Immunology
Volume8
Issue numberMAR
DOIs
Publication statusPublished - Mar 27 2017

Fingerprint

Spleen
Bone Marrow
Radiation
DNA Damage
Radiation Effects
Extracellular Vesicles
MicroRNAs
Cell Count
Bystander Effect
Hematopoietic System
Hematopoietic Stem Cells
Inbred C57BL Mouse
Chromosome Aberrations
DNA Repair
Dendritic Cells
Immune System
X-Rays
Lymphocytes

Keywords

  • Bystander effects
  • Extracellular vesicles
  • Hematopoietic system
  • Ionizing radiation
  • MicroRNA

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Szatmári, T., Kis, D., Bogdándi, E. N., Benedek, A., Bright, S., Bowler, D., ... Lumniczky, K. (2017). Extracellular vesicles mediate radiation-induced systemic bystander signals in the bone marrow and spleen. Frontiers in Immunology, 8(MAR), [347]. https://doi.org/10.3389/fimmu.2017.00347

Extracellular vesicles mediate radiation-induced systemic bystander signals in the bone marrow and spleen. / Szatmári, Tünde; Kis, Dávid; Bogdándi, Eniko Noémi; Benedek, Anett; Bright, Scott; Bowler, Deborah; Persa, Eszter; Kis, Eniko; Balogh, Andrea; Naszályi, Lívia N.; Kadhim, Munira; Sáfrány, G.; Lumniczky, Katalin.

In: Frontiers in Immunology, Vol. 8, No. MAR, 347, 27.03.2017.

Research output: Contribution to journalArticle

Szatmári, T, Kis, D, Bogdándi, EN, Benedek, A, Bright, S, Bowler, D, Persa, E, Kis, E, Balogh, A, Naszályi, LN, Kadhim, M, Sáfrány, G & Lumniczky, K 2017, 'Extracellular vesicles mediate radiation-induced systemic bystander signals in the bone marrow and spleen', Frontiers in Immunology, vol. 8, no. MAR, 347. https://doi.org/10.3389/fimmu.2017.00347
Szatmári, Tünde ; Kis, Dávid ; Bogdándi, Eniko Noémi ; Benedek, Anett ; Bright, Scott ; Bowler, Deborah ; Persa, Eszter ; Kis, Eniko ; Balogh, Andrea ; Naszályi, Lívia N. ; Kadhim, Munira ; Sáfrány, G. ; Lumniczky, Katalin. / Extracellular vesicles mediate radiation-induced systemic bystander signals in the bone marrow and spleen. In: Frontiers in Immunology. 2017 ; Vol. 8, No. MAR.
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