Extracellular vesicles mediate low dose ionizing radiation-induced immune and inflammatory responses in the blood

Tünde Szatmári, Eszter Persa, Enikő Kis, Anett Benedek, Rita Hargitai, G. Sáfrány, Katalin Lumniczky

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Purpose: Radiation-induced bystander effects (RIBE) imply the involvement of complex signaling mechanisms, which can be mediated by extracellular vesicles (EVs). Using an in vivo model, we investigated EV-transmitted RIBE in blood plasma and radiation effects on plasma EV miRNA profiles. Materials and methods: C57Bl/6 mice were total-body irradiated with 0.1 and 2 Gy, bone marrow-derived EVs were isolated, and injected systemically into naive, ‘bystander’ animals. Proteome profiler antibody array membranes were used to detect alterations in plasma, both in directly irradiated and bystander mice. MiRNA profile of plasma EVs was determined by PCR array. Results: M-CSF and pentraxin-3 levels were increased in the blood of directly irradiated and bystander mice both after low and high dose irradiations, CXCL16 and lipocalin-2 increased after 2 Gy in directly irradiated and bystander mice, CCL5 and CCL11 changed in bystander mice only. Substantial overlap was found in the cellular pathways regulated by those miRNAs whose level were altered in EVs isolated from the plasma of mice irradiated with 0.1 and 2 Gy. Several of these pathways have already been associated with bystander responses. Conclusion: Low and high dose effects overlapped both in EV-mediated alterations in signaling pathways leading to RIBE and in their systemic manifestations.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalInternational Journal of Radiation Biology
DOIs
Publication statusAccepted/In press - Mar 29 2018

Fingerprint

Ionizing Radiation
Bystander Effect
Radiation
MicroRNAs
Macrophage Colony-Stimulating Factor
Radiation Effects
Proteome
Extracellular Vesicles
Bone Marrow
Polymerase Chain Reaction
Membranes
Antibodies

Keywords

  • DoReMi
  • extracellular vesicle
  • immune response
  • low dose irradiation
  • microRNA
  • radiation-induced bystander effects

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

Cite this

Extracellular vesicles mediate low dose ionizing radiation-induced immune and inflammatory responses in the blood. / Szatmári, Tünde; Persa, Eszter; Kis, Enikő; Benedek, Anett; Hargitai, Rita; Sáfrány, G.; Lumniczky, Katalin.

In: International Journal of Radiation Biology, 29.03.2018, p. 1-11.

Research output: Contribution to journalArticle

Szatmári, Tünde ; Persa, Eszter ; Kis, Enikő ; Benedek, Anett ; Hargitai, Rita ; Sáfrány, G. ; Lumniczky, Katalin. / Extracellular vesicles mediate low dose ionizing radiation-induced immune and inflammatory responses in the blood. In: International Journal of Radiation Biology. 2018 ; pp. 1-11.
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