Extracellular matrix dynamics during vertebrate axis formation

A. Czirók, Brenda J. Rongish, Charles D. Little

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The first evidence for the dynamics of in vivo extracellular matrix (ECM) pattern formation during embryogenesis is presented below. Fibrillin 2 filaments were tracked for 12 h throughout the avian intraembryonic mesoderm using automated light microscopy and algorithms of our design. The data show that these ECM filaments have a reproducible morphogenic destiny that is characterized by directed transport. Fibrillin 2 particles initially deposited in the segmental plate mesoderm are translocated along an unexpected trajectory where they eventually polymerize into an intricate scaffold of cables parallel to the anterior-posterior axis. The cables coalesce near the midline before the appearance of the next-formed somite. Moreover, the ECM filaments define global tissue movements with high precision because the filaments act as passive motion tracers. Quantification of individual and collective filament "behaviors" establish fate maps, trajectories, and velocities. These data reveal a caudally propagating traveling wave pattern in the morphogenetic movements of early axis formation. We conjecture that within vertebrate embryos, long-range mechanical tension fields are coupled to both large-scale patterning and local organization of the ECM. Thus, physical forces or stress fields are essential requirements for executing an emergent developmental pattern - in this case, paraxial fibrillin cable assembly.

Original languageEnglish
Pages (from-to)111-122
Number of pages12
JournalDevelopmental Biology
Volume268
Issue number1
DOIs
Publication statusPublished - Apr 1 2004

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Extracellular Matrix
Vertebrates
Mesoderm
Somites
Embryonic Development
Microscopy
Embryonic Structures
Light
Fibrillin-2

Keywords

  • Dynamics
  • Extracellular matrix
  • Fate map
  • Gastrulation
  • Time-lapse
  • Tissue deformation

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Extracellular matrix dynamics during vertebrate axis formation. / Czirók, A.; Rongish, Brenda J.; Little, Charles D.

In: Developmental Biology, Vol. 268, No. 1, 01.04.2004, p. 111-122.

Research output: Contribution to journalArticle

Czirók, A. ; Rongish, Brenda J. ; Little, Charles D. / Extracellular matrix dynamics during vertebrate axis formation. In: Developmental Biology. 2004 ; Vol. 268, No. 1. pp. 111-122.
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