Extent and properties of nonbulk 'bound' water in crystalline lens cells

I. L. Cameron, E. Contreras, G. D. Fullerton, M. Kellermayer, A. Ludany, A. Miseta

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Crystalline lenses provided good material to study and measure the properties of cellular water. Different methods were used to establish the extent and properties of nonbulk water in mammalian lenses. These methods include: NMR titration analysis, a test of the osmotic properties, a test of dye exclusion. In lenses with intact cell membranes and in lenses with disrupted cell membranes, and the water-holding capacity of lenses subjected to 40,000 x g for 1 hour with intact cell membranes and in lenses with disrupted cell membranes. The data from these methods, as well as other data from the literature, lead to the conclusion that most, if not all, of the water in lens cells (up to 2.2 g water/g dry mass) has motional and osmotic properties that distinguish it from bulk water. These findings call into question the common and convenient assumption that all but a small proportion of cellular water is like that in dilute solution.

Original languageEnglish
Pages (from-to)125-132
Number of pages8
JournalJournal of Cellular Physiology
Volume137
Issue number1
Publication statusPublished - 1988

Fingerprint

Crystalline Lens
Lenses
Crystalline materials
Cell membranes
Water
Cell Membrane
Titration
Coloring Agents
Nuclear magnetic resonance

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Cameron, I. L., Contreras, E., Fullerton, G. D., Kellermayer, M., Ludany, A., & Miseta, A. (1988). Extent and properties of nonbulk 'bound' water in crystalline lens cells. Journal of Cellular Physiology, 137(1), 125-132.

Extent and properties of nonbulk 'bound' water in crystalline lens cells. / Cameron, I. L.; Contreras, E.; Fullerton, G. D.; Kellermayer, M.; Ludany, A.; Miseta, A.

In: Journal of Cellular Physiology, Vol. 137, No. 1, 1988, p. 125-132.

Research output: Contribution to journalArticle

Cameron, IL, Contreras, E, Fullerton, GD, Kellermayer, M, Ludany, A & Miseta, A 1988, 'Extent and properties of nonbulk 'bound' water in crystalline lens cells', Journal of Cellular Physiology, vol. 137, no. 1, pp. 125-132.
Cameron IL, Contreras E, Fullerton GD, Kellermayer M, Ludany A, Miseta A. Extent and properties of nonbulk 'bound' water in crystalline lens cells. Journal of Cellular Physiology. 1988;137(1):125-132.
Cameron, I. L. ; Contreras, E. ; Fullerton, G. D. ; Kellermayer, M. ; Ludany, A. ; Miseta, A. / Extent and properties of nonbulk 'bound' water in crystalline lens cells. In: Journal of Cellular Physiology. 1988 ; Vol. 137, No. 1. pp. 125-132.
@article{a8e31c2b61be43a6a78e74db4e899eb5,
title = "Extent and properties of nonbulk 'bound' water in crystalline lens cells",
abstract = "Crystalline lenses provided good material to study and measure the properties of cellular water. Different methods were used to establish the extent and properties of nonbulk water in mammalian lenses. These methods include: NMR titration analysis, a test of the osmotic properties, a test of dye exclusion. In lenses with intact cell membranes and in lenses with disrupted cell membranes, and the water-holding capacity of lenses subjected to 40,000 x g for 1 hour with intact cell membranes and in lenses with disrupted cell membranes. The data from these methods, as well as other data from the literature, lead to the conclusion that most, if not all, of the water in lens cells (up to 2.2 g water/g dry mass) has motional and osmotic properties that distinguish it from bulk water. These findings call into question the common and convenient assumption that all but a small proportion of cellular water is like that in dilute solution.",
author = "Cameron, {I. L.} and E. Contreras and Fullerton, {G. D.} and M. Kellermayer and A. Ludany and A. Miseta",
year = "1988",
language = "English",
volume = "137",
pages = "125--132",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Extent and properties of nonbulk 'bound' water in crystalline lens cells

AU - Cameron, I. L.

AU - Contreras, E.

AU - Fullerton, G. D.

AU - Kellermayer, M.

AU - Ludany, A.

AU - Miseta, A.

PY - 1988

Y1 - 1988

N2 - Crystalline lenses provided good material to study and measure the properties of cellular water. Different methods were used to establish the extent and properties of nonbulk water in mammalian lenses. These methods include: NMR titration analysis, a test of the osmotic properties, a test of dye exclusion. In lenses with intact cell membranes and in lenses with disrupted cell membranes, and the water-holding capacity of lenses subjected to 40,000 x g for 1 hour with intact cell membranes and in lenses with disrupted cell membranes. The data from these methods, as well as other data from the literature, lead to the conclusion that most, if not all, of the water in lens cells (up to 2.2 g water/g dry mass) has motional and osmotic properties that distinguish it from bulk water. These findings call into question the common and convenient assumption that all but a small proportion of cellular water is like that in dilute solution.

AB - Crystalline lenses provided good material to study and measure the properties of cellular water. Different methods were used to establish the extent and properties of nonbulk water in mammalian lenses. These methods include: NMR titration analysis, a test of the osmotic properties, a test of dye exclusion. In lenses with intact cell membranes and in lenses with disrupted cell membranes, and the water-holding capacity of lenses subjected to 40,000 x g for 1 hour with intact cell membranes and in lenses with disrupted cell membranes. The data from these methods, as well as other data from the literature, lead to the conclusion that most, if not all, of the water in lens cells (up to 2.2 g water/g dry mass) has motional and osmotic properties that distinguish it from bulk water. These findings call into question the common and convenient assumption that all but a small proportion of cellular water is like that in dilute solution.

UR - http://www.scopus.com/inward/record.url?scp=0023784658&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023784658&partnerID=8YFLogxK

M3 - Article

C2 - 3170652

AN - SCOPUS:0023784658

VL - 137

SP - 125

EP - 132

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

IS - 1

ER -