Extension of the CD4+Foxp3+CD25-/low regulatory T-cell subpopulation in type 1 diabetes mellitus

András Zóka, G. Barna, A. Somogyi, G. Müzes, Ágnes Oláh, Zahra Al-Aissa, Orsolya Hadarits, Katalin Kiss, G. Firneisz

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Regulatory T-cells (Treg) have a crucial role in limiting physiologic autoreactivity. Foxp3 is a master regulator transcription factor of Treg differentiation and active Treg cells express high levels of IL-2 receptor α-chain (CD25). The aim of our study was to assess the key markers of Treg cell function in type 1 diabetic (T1DM) and control subjects by flow cytometry. The proportion of CD25-/low cells among CD4+Foxp3+ Treg cells was higher in T1DM patients that might suggest a shifted proportion of the incomplete/reserve and the fully active (CD4+Foxp3+CD25+) Treg cell subpopulations in T1DM, similarly to other Th1-mediated autoimmune diseases. In addition to the decreased expression of CD25 and CTLA-4 in T1DM patients, a positive correlation was observed between the CD25 expression on CD4+ and the CTLA-4 expression in CD8- T-lymphocytes both in the T1DM and in the healthy control group. Our results suggest an impaired balance of CD25+ and CD25-/low Treg cells in T1DM which might reflect a decreased late phase peripheral Treg activation even in patients with a mean disease duration of more than a decade.

Original languageEnglish
Pages (from-to)289-297
Number of pages9
JournalAutoimmunity
Volume48
Issue number5
DOIs
Publication statusPublished - Aug 1 2015

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Regulatory T-Lymphocytes
Type 1 Diabetes Mellitus
Interleukin-2 Receptors
Autoimmune Diseases
Flow Cytometry
Transcription Factors
T-Lymphocytes
Control Groups

Keywords

  • Autoreactivity
  • CD25
  • Foxp3
  • Immune regulation
  • Insulitis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Extension of the CD4+Foxp3+CD25-/low regulatory T-cell subpopulation in type 1 diabetes mellitus. / Zóka, András; Barna, G.; Somogyi, A.; Müzes, G.; Oláh, Ágnes; Al-Aissa, Zahra; Hadarits, Orsolya; Kiss, Katalin; Firneisz, G.

In: Autoimmunity, Vol. 48, No. 5, 01.08.2015, p. 289-297.

Research output: Contribution to journalArticle

Zóka, András ; Barna, G. ; Somogyi, A. ; Müzes, G. ; Oláh, Ágnes ; Al-Aissa, Zahra ; Hadarits, Orsolya ; Kiss, Katalin ; Firneisz, G. / Extension of the CD4+Foxp3+CD25-/low regulatory T-cell subpopulation in type 1 diabetes mellitus. In: Autoimmunity. 2015 ; Vol. 48, No. 5. pp. 289-297.
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