Extensibility of the extended tail domain of processive and nonprocessive myosin V molecules

A. Nagy, Grzegorz Piszczek, James R. Sellers

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Myosin V is a single-molecule motor that moves organelles along actin. When myosin V pulls loads inside the cell in a highly viscous environment, the force on the motor is unlikely to be constant. We propose that the tether between the single-molecule motor and the cargo (i.e., the extended tail domain of the molecule) must be able to absorb the sudden mechanical motions of the motor and allow smooth relaxation of the motion of the cargo to a new position. To test this hypothesis, we compared the elastic properties of the extended tail domains of processive (mouse myosin Va) and nonprocessive (Drosophila myosin V) molecular motors. The extended tail domain of these myosins consists of mechanically strong coiled-coil regions interspersed with flexible loops. In this work we explored the mechanical properties of coiled-coil regions using atomic force microscopy. We found that the processive and nonprocessive coiled-coil fragments display different unfolding patterns. The unfolding of coiled-coil structures occurs much later during the atomic force microscopy stretch cycle for processive myosin Va than for nonprocessive Drosophila myosin V, suggesting that this elastic tether between the cargo and motor may play an important role in sustaining the processive motions of this single-molecule motor.

Original languageEnglish
Pages (from-to)3123-3131
Number of pages9
JournalBiophysical Journal
Volume97
Issue number12
DOIs
Publication statusPublished - Dec 16 2009

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Myosin Type V
Tail
Myosins
Atomic Force Microscopy
Drosophila
Organelles
Actins

ASJC Scopus subject areas

  • Biophysics

Cite this

Extensibility of the extended tail domain of processive and nonprocessive myosin V molecules. / Nagy, A.; Piszczek, Grzegorz; Sellers, James R.

In: Biophysical Journal, Vol. 97, No. 12, 16.12.2009, p. 3123-3131.

Research output: Contribution to journalArticle

Nagy, A. ; Piszczek, Grzegorz ; Sellers, James R. / Extensibility of the extended tail domain of processive and nonprocessive myosin V molecules. In: Biophysical Journal. 2009 ; Vol. 97, No. 12. pp. 3123-3131.
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