Expression vectors based on the rac fusion promoter

Imre Boros, Tamas Lukacsovich, Gabriella Baliko, Pal Venetianer

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The -35 region of the rrnB P2 promoter and the -10 region of the lacZpo promoter-operator were fused to form the strong and regulatable rac promoter. Vectors were constructed that allow the attachment of protein-coding sequences to the β-galactosidase α-peptide (LacZα) in any reading frame. By introducing a high-copy-number mutation, the synthesis of a LacZ α-chloramphenicol acetyltransferase fusion protein reached more than 60% of total cell protein in Escherichia coli.

Original languageEnglish
Pages (from-to)97-100
Number of pages4
JournalGene
Volume42
Issue number1
DOIs
Publication statusPublished - 1986

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Keywords

  • Recombinant DNA
  • chloramphenicol acetyltransferase
  • high-copy-number
  • lac promoter
  • pBR322 plasmid
  • rrnB P promoter

ASJC Scopus subject areas

  • Genetics

Cite this

Boros, I., Lukacsovich, T., Baliko, G., & Venetianer, P. (1986). Expression vectors based on the rac fusion promoter. Gene, 42(1), 97-100. https://doi.org/10.1016/0378-1119(86)90154-X