Expression vectors and gene fusions for the directed modification of the carotenoid biosynthesis pathway in Mucor circinelloides

Enrique A. Iturriaga, María Isabel Alvarez, Arturo P. Eslava, T. Papp

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Several fungal species, particularly some included in the Mucoromycotina, have been used to develop fermentation processes for the production of β-carotene. Oxygenated derivatives of β-carotene (xanthophylls) are desirable value-added products, and the preference by the market of carotenoids from biological sources has increased the research in different carotenoid-producing organisms. We currently use Mucor circinelloides f. lusitanicus as a model organism to develop strains with an increased content of new and more valuable carotenoids. The main carotenoid accumulated by M. circinelloides is β-carotene, although it has some hydroxylase activity and produces low amounts of zeaxanthin. On the other hand, in astaxanthin-producing organisms two enzymatic activities are required for the production of astaxanthin from β-carotene: a hydroxylase and a ketolase. In this chapter, we delineate part of our efforts to construct genetically modified strains that could advance in the improvement of carotenoid accumulation by this fungus and the diversification of its carotenoid content. Accordingly, we describe detailed and empirically tested protocols for the construction of functional expression vectors and gene fusions.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages239-256
Number of pages18
DOIs
Publication statusPublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1852
ISSN (Print)1064-3745

Fingerprint

Mucor
Gene Fusion
Carotenoids
Mixed Function Oxygenases
Xanthophylls
Fermentation
Fungi

Keywords

  • Astaxanthin
  • Expression vectors
  • Gene fusions
  • Metabolic engineering
  • Mucor
  • Xanthophylls

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Iturriaga, E. A., Alvarez, M. I., Eslava, A. P., & Papp, T. (2018). Expression vectors and gene fusions for the directed modification of the carotenoid biosynthesis pathway in Mucor circinelloides. In Methods in Molecular Biology (pp. 239-256). (Methods in Molecular Biology; Vol. 1852). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8742-9_14

Expression vectors and gene fusions for the directed modification of the carotenoid biosynthesis pathway in Mucor circinelloides. / Iturriaga, Enrique A.; Alvarez, María Isabel; Eslava, Arturo P.; Papp, T.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 239-256 (Methods in Molecular Biology; Vol. 1852).

Research output: Chapter in Book/Report/Conference proceedingChapter

Iturriaga, EA, Alvarez, MI, Eslava, AP & Papp, T 2018, Expression vectors and gene fusions for the directed modification of the carotenoid biosynthesis pathway in Mucor circinelloides. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1852, Humana Press Inc., pp. 239-256. https://doi.org/10.1007/978-1-4939-8742-9_14
Iturriaga EA, Alvarez MI, Eslava AP, Papp T. Expression vectors and gene fusions for the directed modification of the carotenoid biosynthesis pathway in Mucor circinelloides. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 239-256. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8742-9_14
Iturriaga, Enrique A. ; Alvarez, María Isabel ; Eslava, Arturo P. ; Papp, T. / Expression vectors and gene fusions for the directed modification of the carotenoid biosynthesis pathway in Mucor circinelloides. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 239-256 (Methods in Molecular Biology).
@inbook{7e27fc0af4c94fa1a79cb446868e7500,
title = "Expression vectors and gene fusions for the directed modification of the carotenoid biosynthesis pathway in Mucor circinelloides",
abstract = "Several fungal species, particularly some included in the Mucoromycotina, have been used to develop fermentation processes for the production of β-carotene. Oxygenated derivatives of β-carotene (xanthophylls) are desirable value-added products, and the preference by the market of carotenoids from biological sources has increased the research in different carotenoid-producing organisms. We currently use Mucor circinelloides f. lusitanicus as a model organism to develop strains with an increased content of new and more valuable carotenoids. The main carotenoid accumulated by M. circinelloides is β-carotene, although it has some hydroxylase activity and produces low amounts of zeaxanthin. On the other hand, in astaxanthin-producing organisms two enzymatic activities are required for the production of astaxanthin from β-carotene: a hydroxylase and a ketolase. In this chapter, we delineate part of our efforts to construct genetically modified strains that could advance in the improvement of carotenoid accumulation by this fungus and the diversification of its carotenoid content. Accordingly, we describe detailed and empirically tested protocols for the construction of functional expression vectors and gene fusions.",
keywords = "Astaxanthin, Expression vectors, Gene fusions, Metabolic engineering, Mucor, Xanthophylls",
author = "Iturriaga, {Enrique A.} and Alvarez, {Mar{\'i}a Isabel} and Eslava, {Arturo P.} and T. Papp",
year = "2018",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-8742-9_14",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "239--256",
booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Expression vectors and gene fusions for the directed modification of the carotenoid biosynthesis pathway in Mucor circinelloides

AU - Iturriaga, Enrique A.

AU - Alvarez, María Isabel

AU - Eslava, Arturo P.

AU - Papp, T.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Several fungal species, particularly some included in the Mucoromycotina, have been used to develop fermentation processes for the production of β-carotene. Oxygenated derivatives of β-carotene (xanthophylls) are desirable value-added products, and the preference by the market of carotenoids from biological sources has increased the research in different carotenoid-producing organisms. We currently use Mucor circinelloides f. lusitanicus as a model organism to develop strains with an increased content of new and more valuable carotenoids. The main carotenoid accumulated by M. circinelloides is β-carotene, although it has some hydroxylase activity and produces low amounts of zeaxanthin. On the other hand, in astaxanthin-producing organisms two enzymatic activities are required for the production of astaxanthin from β-carotene: a hydroxylase and a ketolase. In this chapter, we delineate part of our efforts to construct genetically modified strains that could advance in the improvement of carotenoid accumulation by this fungus and the diversification of its carotenoid content. Accordingly, we describe detailed and empirically tested protocols for the construction of functional expression vectors and gene fusions.

AB - Several fungal species, particularly some included in the Mucoromycotina, have been used to develop fermentation processes for the production of β-carotene. Oxygenated derivatives of β-carotene (xanthophylls) are desirable value-added products, and the preference by the market of carotenoids from biological sources has increased the research in different carotenoid-producing organisms. We currently use Mucor circinelloides f. lusitanicus as a model organism to develop strains with an increased content of new and more valuable carotenoids. The main carotenoid accumulated by M. circinelloides is β-carotene, although it has some hydroxylase activity and produces low amounts of zeaxanthin. On the other hand, in astaxanthin-producing organisms two enzymatic activities are required for the production of astaxanthin from β-carotene: a hydroxylase and a ketolase. In this chapter, we delineate part of our efforts to construct genetically modified strains that could advance in the improvement of carotenoid accumulation by this fungus and the diversification of its carotenoid content. Accordingly, we describe detailed and empirically tested protocols for the construction of functional expression vectors and gene fusions.

KW - Astaxanthin

KW - Expression vectors

KW - Gene fusions

KW - Metabolic engineering

KW - Mucor

KW - Xanthophylls

UR - http://www.scopus.com/inward/record.url?scp=85051824701&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85051824701&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-8742-9_14

DO - 10.1007/978-1-4939-8742-9_14

M3 - Chapter

C2 - 30109635

AN - SCOPUS:85051824701

T3 - Methods in Molecular Biology

SP - 239

EP - 256

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -