Expression patterns of ethylene and polyamine biosynthetic genes during fruit ripening in strawberry

Ákos Mendel, László Kovács, Anna Szentgyörgyi, S. Fekete, Katalin Posta, E. Kiss

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Abstract

The expression pattern of genes related to ethylene and polyamine biosynthesis were analyzed in octoploid strawberry fruits in different ripening phases. RNA was isolated from Fragaria x ananassa Duch cv. Asia and used to synthesize cDNA by reverse transcriptase enzyme, which was applied in qPCR with primers designed for S-adenosyl-L-methionine synthases (FaSAMS1-3), S-adenosyl-L-methionine decarboxylases (FaSAMDC1-3), arginine decarboxylase (FaADC), spermidine synthase (FaSPDS), 1-aminocyclopropane-1-carboxylate synthases (FaACS1-4) and 1-aminocyclopropane-1-carboxylate oxidase (FaACO1-4) gene sequences. Based on the comparison made between the expression patterns of ethylene- and polyamine-related genes and those of the common precursor- and pathway shift-related genes, it appeared that with the exception of FaSAMS2, FaSAMDC1 and FaSAMDC2, the genes involved in polyamine biosynthesis showed decreasing level of expression as ripening proceeded.

Original languageEnglish
Pages (from-to)174-182
Number of pages9
JournalStudia Universitatis Vasile Goldis Arad, Seria Stiintele Vietii
Volume28
Issue number4
Publication statusPublished - Jan 1 2018

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Keywords

  • ACC oxidase
  • ACC synthase
  • S-adenosyl-L-methionine
  • SAM decarboxylase
  • Spermidine synthase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Agricultural and Biological Sciences(all)

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