Expression pattern of the human ABC transporters in pluripotent embryonic stem cells and in their derivatives

Zsuzsa Erdei, Réka Lorincz, Kornélia Szebényi, Adrienn Péntek, Nóra Varga, István Likó, György Várady, G. Szakács, Tamás I. Orbán, B. Sarkadi, A. Apáti

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background ATP-binding cassette (ABC) transporters have key roles in various physiological functions as well as providing chemical defense and stress tolerance in human tissues. In this study, we have examined the expression pattern of all ABC proteins in pluripotent human embryonic stem cells (hESCs) and in their differentiated progenies. We paid special attention to the cellular expression and localization of multidrug transporter ABC proteins. Methods Stem cell differentiation was carried out without chemical induction or cell sorting, and specialized cell types were separated mechanically. Cellular features regarding pluripotency and tissue identity, as well as ABC transporter expression were studied by flow cytomtery, immuno-microscopy, and qPCR-based low-density arrays. Results Pluripotent hESCs and differentiated cell types (cardiomyocytes, neuronal cells, and mesenchymal stem cells) were distinguished by morphology, immunostaining markers, and selected mRNA expression patterns. We found that the mRNA expression levels of the 48 human ABC proteins also clearly distinguished the pluripotent and the respective differentiated cell types. When multidrug and lipid transporter ABC protein expression was examined by using well characterized specific antibodies by flow cytometry and confocal microscopy, the protein expression data corresponded well to the mRNA expression results. Moreover, the cellular localization of these important human ABC transporter proteins could be established in the pluripotent and differentiated hESC derived samples. Conclusions These studies provide valuable information regarding ABC protein expression in human stem cells and their differentiated offspring. The results may also help to obtain further information concerning the specialized cellular functions of selected ABC transporters.

Original languageEnglish
Pages (from-to)299-310
Number of pages12
JournalCytometry Part B - Clinical Cytometry
Volume86
Issue number5
DOIs
Publication statusPublished - 2014

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Pluripotent Stem Cells
ATP-Binding Cassette Transporters
Embryonic Stem Cells
Carrier Proteins
Adenosine Triphosphate
Messenger RNA
Stem Cells
Mesenchymal Stromal Cells
Cardiac Myocytes
Confocal Microscopy
Cell Differentiation
Microscopy
Flow Cytometry
Proteins
Lipids
Antibodies
Human Embryonic Stem Cells

Keywords

  • ABC transporters
  • cardiomyocyte
  • flow cytometry
  • human pluripotent stem cell
  • mesenchymal cell
  • microscopy
  • neural cells
  • pluripotency
  • stem cells

ASJC Scopus subject areas

  • Cell Biology
  • Histology
  • Pathology and Forensic Medicine
  • Medicine(all)

Cite this

Expression pattern of the human ABC transporters in pluripotent embryonic stem cells and in their derivatives. / Erdei, Zsuzsa; Lorincz, Réka; Szebényi, Kornélia; Péntek, Adrienn; Varga, Nóra; Likó, István; Várady, György; Szakács, G.; Orbán, Tamás I.; Sarkadi, B.; Apáti, A.

In: Cytometry Part B - Clinical Cytometry, Vol. 86, No. 5, 2014, p. 299-310.

Research output: Contribution to journalArticle

Erdei, Zsuzsa ; Lorincz, Réka ; Szebényi, Kornélia ; Péntek, Adrienn ; Varga, Nóra ; Likó, István ; Várady, György ; Szakács, G. ; Orbán, Tamás I. ; Sarkadi, B. ; Apáti, A. / Expression pattern of the human ABC transporters in pluripotent embryonic stem cells and in their derivatives. In: Cytometry Part B - Clinical Cytometry. 2014 ; Vol. 86, No. 5. pp. 299-310.
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AU - Lorincz, Réka

AU - Szebényi, Kornélia

AU - Péntek, Adrienn

AU - Varga, Nóra

AU - Likó, István

AU - Várady, György

AU - Szakács, G.

AU - Orbán, Tamás I.

AU - Sarkadi, B.

AU - Apáti, A.

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N2 - Background ATP-binding cassette (ABC) transporters have key roles in various physiological functions as well as providing chemical defense and stress tolerance in human tissues. In this study, we have examined the expression pattern of all ABC proteins in pluripotent human embryonic stem cells (hESCs) and in their differentiated progenies. We paid special attention to the cellular expression and localization of multidrug transporter ABC proteins. Methods Stem cell differentiation was carried out without chemical induction or cell sorting, and specialized cell types were separated mechanically. Cellular features regarding pluripotency and tissue identity, as well as ABC transporter expression were studied by flow cytomtery, immuno-microscopy, and qPCR-based low-density arrays. Results Pluripotent hESCs and differentiated cell types (cardiomyocytes, neuronal cells, and mesenchymal stem cells) were distinguished by morphology, immunostaining markers, and selected mRNA expression patterns. We found that the mRNA expression levels of the 48 human ABC proteins also clearly distinguished the pluripotent and the respective differentiated cell types. When multidrug and lipid transporter ABC protein expression was examined by using well characterized specific antibodies by flow cytometry and confocal microscopy, the protein expression data corresponded well to the mRNA expression results. Moreover, the cellular localization of these important human ABC transporter proteins could be established in the pluripotent and differentiated hESC derived samples. Conclusions These studies provide valuable information regarding ABC protein expression in human stem cells and their differentiated offspring. The results may also help to obtain further information concerning the specialized cellular functions of selected ABC transporters.

AB - Background ATP-binding cassette (ABC) transporters have key roles in various physiological functions as well as providing chemical defense and stress tolerance in human tissues. In this study, we have examined the expression pattern of all ABC proteins in pluripotent human embryonic stem cells (hESCs) and in their differentiated progenies. We paid special attention to the cellular expression and localization of multidrug transporter ABC proteins. Methods Stem cell differentiation was carried out without chemical induction or cell sorting, and specialized cell types were separated mechanically. Cellular features regarding pluripotency and tissue identity, as well as ABC transporter expression were studied by flow cytomtery, immuno-microscopy, and qPCR-based low-density arrays. Results Pluripotent hESCs and differentiated cell types (cardiomyocytes, neuronal cells, and mesenchymal stem cells) were distinguished by morphology, immunostaining markers, and selected mRNA expression patterns. We found that the mRNA expression levels of the 48 human ABC proteins also clearly distinguished the pluripotent and the respective differentiated cell types. When multidrug and lipid transporter ABC protein expression was examined by using well characterized specific antibodies by flow cytometry and confocal microscopy, the protein expression data corresponded well to the mRNA expression results. Moreover, the cellular localization of these important human ABC transporter proteins could be established in the pluripotent and differentiated hESC derived samples. Conclusions These studies provide valuable information regarding ABC protein expression in human stem cells and their differentiated offspring. The results may also help to obtain further information concerning the specialized cellular functions of selected ABC transporters.

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