Expression pattern of Filamin-240 in Drosophila blood cells

Florentina Rus, E. Kurucz, Róbert Márkus, Sergey A. Sinenko, Barbara Laurinyecz, Csilla Pataki, János Gausz, Z. Hegedűs, A. Udvardy, Dan Hultmark, I. Andó

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The expression pattern of Filamin-240 was studied in subsets of Drosophila blood cells by means of immunofluorescent staining and Western blot analysis with use of an antibody specific to a "filamin-folding domain", a consensus motif profile generated from the 20 existing filamin repeats. Expression of Filamin-240 is restricted to lamellocytes - a special blood cell type of the cellular immune response - and is involved in the regulation of lamellocyte development. In the cher1 homozygous larvae, which lack Filamin-240 protein, a vigorous lamellocyte differentiation occurs which is further enhanced upon in vivo immune challenge by a parasitic wasp, Leptopilina boulardi. By introducing a full-length transgene encoding the Drosophila Filamin-240 protein into the cher1 Filamin-deficient homozygous mutant, the mutant blood cell phenotype was rescued. These data demonstrate that the expression of Filamin-240 is strictly lamellocyte specific in Drosophila blood cells and that the protein is a suppressor of lamellocyte development.

Original languageEnglish
Pages (from-to)928-934
Number of pages7
JournalGene Expression Patterns
Volume6
Issue number8
DOIs
Publication statusPublished - Oct 2 2006

Fingerprint

Filamins
Drosophila
Blood Cells
Drosophila Proteins
Wasps
Transgenes
Cellular Immunity
Larva
Blood Proteins
Western Blotting
Staining and Labeling
Phenotype
Antibodies

Keywords

  • Actin network
  • Blood cell
  • Blood cell development
  • Drosophila
  • Encapsulation
  • Filamin
  • Hemocyte
  • Immune induction
  • Lamellocyte

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Expression pattern of Filamin-240 in Drosophila blood cells. / Rus, Florentina; Kurucz, E.; Márkus, Róbert; Sinenko, Sergey A.; Laurinyecz, Barbara; Pataki, Csilla; Gausz, János; Hegedűs, Z.; Udvardy, A.; Hultmark, Dan; Andó, I.

In: Gene Expression Patterns, Vol. 6, No. 8, 02.10.2006, p. 928-934.

Research output: Contribution to journalArticle

Rus, F, Kurucz, E, Márkus, R, Sinenko, SA, Laurinyecz, B, Pataki, C, Gausz, J, Hegedűs, Z, Udvardy, A, Hultmark, D & Andó, I 2006, 'Expression pattern of Filamin-240 in Drosophila blood cells', Gene Expression Patterns, vol. 6, no. 8, pp. 928-934. https://doi.org/10.1016/j.modgep.2006.03.005
Rus, Florentina ; Kurucz, E. ; Márkus, Róbert ; Sinenko, Sergey A. ; Laurinyecz, Barbara ; Pataki, Csilla ; Gausz, János ; Hegedűs, Z. ; Udvardy, A. ; Hultmark, Dan ; Andó, I. / Expression pattern of Filamin-240 in Drosophila blood cells. In: Gene Expression Patterns. 2006 ; Vol. 6, No. 8. pp. 928-934.
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