Expression pattern of clock under acute phase-delay of the light/dark cycle in the chicken pineal model

Siri Kommedal, Gergely Bódis, Attila Matkovits, V. Csernus, András D. Nagy

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Shift workers have a higher risk of metabolic syndrome, a condition that also develops in mice carrying mutation in their circadian clock gene clock. To collect more data on the transcriptional changes of clock under phase-shifted light/dark LD conditions, we examined the 24. h patterns of clock mRNA expression in vivo and in vitro in chickens exposed acutely to a reversed LD (DL) cycle. Under controlled LD conditions (lights on at 6:00, lights off at 20:00), clock mRNA expression peaked in vivo at 2:00 (Zeitgeber Time 20, ZT20) and in vitro at 22:00 (ZT16). Even higher mRNA contents were measured in the first cycle of in vivo DL conditions between 22:00 and 6:00 (lights at night), but in the second cycle by 2:00, lower mRNA contents were detected than the control peak values seen at this time point. Furthermore, no alterations were found in vitro in clock mRNA content during the first 12. h of DL conditions (lights at night). The differences seen between the first and the second DL cycles in vivo and between the in vivo and in vitro data for the first DL cycle support the idea that neurohumoral signals perturbed by a phase-delayed light-dark cycle may also play a role in the in vivo rapid transcriptional resetting of the circadian clock in the chicken pineal model.

Original languageEnglish
Pages (from-to)170-172
Number of pages3
JournalGeneral and Comparative Endocrinology
Volume172
Issue number1
DOIs
Publication statusPublished - May 15 2011

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Photoperiod
Chickens
photoperiod
chickens
Light
Messenger RNA
Circadian Clocks
circadian rhythm
metabolic syndrome
mutation
Mutation
In Vitro Techniques
mice
Genes
genes

Keywords

  • Chicken
  • Circadian
  • CLOCK
  • Clock genes
  • Jet lag
  • Pineal gland

ASJC Scopus subject areas

  • Endocrinology

Cite this

Expression pattern of clock under acute phase-delay of the light/dark cycle in the chicken pineal model. / Kommedal, Siri; Bódis, Gergely; Matkovits, Attila; Csernus, V.; Nagy, András D.

In: General and Comparative Endocrinology, Vol. 172, No. 1, 15.05.2011, p. 170-172.

Research output: Contribution to journalArticle

Kommedal, Siri ; Bódis, Gergely ; Matkovits, Attila ; Csernus, V. ; Nagy, András D. / Expression pattern of clock under acute phase-delay of the light/dark cycle in the chicken pineal model. In: General and Comparative Endocrinology. 2011 ; Vol. 172, No. 1. pp. 170-172.
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