Expression of vesicular glutamate transporter-2 in gonadotropin-releasing hormone neurons of the adult male rat

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Abstract

Isoforms of the recently cloned vesicular glutamate transporters (VGLUT1-3) selectively accumulate glutamic acid into synaptic vesicles in excitatory ason terminals and are viewed as reliable markers for glutamatergic neurons. Our present studies provided dual-label in situ hybridization evidence that virtually all (99.5%) GnRH neurons express VGLUT2 mRNA in the preoptic region of the adult male rat. Dual-label immunofluorescent experiments were carried out to examine the presence of VGLUT2 protein in GnRH axon terminals. Confocal laser microscopic analysis of the organum vasculosum of the lamina terminalis and the external zone of the median eminence, the major termination fields for GnRH-secreting axons, demonstrated the frequent occurrence of VGLUT2 immunoreactivity in GnRH axon terminals. Together these mRNA hybridization and immunocytochemical data indicate that GnRH neurons of the adult male rat possess marked glutamatergic characteristics. The physiological significance of endogenous glutamate in the regulation of gonadotropin secretion requires clarification.

Original languageEnglish
Pages (from-to)4018-4021
Number of pages4
JournalEndocrinology
Volume145
Issue number9
DOIs
Publication statusPublished - Sep 2004

Fingerprint

Vesicular Glutamate Transport Protein 2
Gonadotropin-Releasing Hormone
Neurons
Presynaptic Terminals
Glutamic Acid
Vesicular Glutamate Transport Proteins
Messenger RNA
Median Eminence
Synaptic Vesicles
Gonadotropins
In Situ Hybridization
Axons
Protein Isoforms
Lasers

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Expression of vesicular glutamate transporter-2 in gonadotropin-releasing hormone neurons of the adult male rat. / Hrabovszky, E.; Turi, Gergely F.; Kalló, I.; Liposits, Z.

In: Endocrinology, Vol. 145, No. 9, 09.2004, p. 4018-4021.

Research output: Contribution to journalArticle

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