Expression of two metallothionein genes in different brain regions of common CARP

K. Said Ali, Ágnes Ferencz, Aranka K. Deér, J. Nemcsók, E. Hermesz

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The expression pattern of two metallothionein (MT) genes in response to temperature shock and exposure to Cd 2+ was investigated in the brain of common carp ( Cyprinus carpio ), in whole-animal experiments. The changes in the levels of MT-1 and MT-2 mRNA in the olfactory lobe, midbrain and cerebellum were followed by semiquantitative RT-PCR. The inducibility of the two MT genes was brain regionand stressor-specific. Cd 2+ affected mostly the expression of MT-2, while the level of the MT-1 transcript did not change significantly in any of the brain regions examined. Moreover, the MT-2 expression was regulated spatially; MT-2 was induced significantly more strongly in the olfactory lobe than in the cerebellum or midbrain. A sudden temperature drop mainly affected the expression of the MT-1 gene; after 5 h of cold shock, the MT-1 mRNA level was about 25% of the basal value in the cerebellum and the midbrain region. The MT-2 expression did not change significantly during this treatment.

Original languageEnglish
Pages (from-to)149-158
Number of pages10
JournalActa Biologica Hungarica
Volume60
Issue number2
DOIs
Publication statusPublished - Jun 1 2009

Fingerprint

metallothionein
Metallothionein
brain
Brain
Genes
gene
Mesencephalon
Cerebellum
Carps
Shock
Messenger RNA
Temperature
Animals
temperature

Keywords

  • Brain
  • Carp
  • Cd treatment
  • Metallothionein mRNAs
  • RT-PCR

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Expression of two metallothionein genes in different brain regions of common CARP. / Ali, K. Said; Ferencz, Ágnes; Deér, Aranka K.; Nemcsók, J.; Hermesz, E.

In: Acta Biologica Hungarica, Vol. 60, No. 2, 01.06.2009, p. 149-158.

Research output: Contribution to journalArticle

Ali, K. Said ; Ferencz, Ágnes ; Deér, Aranka K. ; Nemcsók, J. ; Hermesz, E. / Expression of two metallothionein genes in different brain regions of common CARP. In: Acta Biologica Hungarica. 2009 ; Vol. 60, No. 2. pp. 149-158.
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