Expression of tight junction molecules in breast carcinomas analysed by array PCR and immunohistochemistry

A. Tőkés, A. Szász, Éva Juhász, Z. Schaff, László Harsányi, István Arthur Molnár, Zsolt Baranyai, István Besznyák, Attila Zaránd, Ferenc Salamon, J. Kulka

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In the past few decades an enormous amount of data became known to clarify the molecular composition and architecture of tight junctions (TJs). Despite the efforts, the expression and function of several TJ genes and proteins in breast carcinoma are still not known and some of the data are contradictory. The expression of forty-four TJ associated genes was examined at mRNA level in eighteen invasive ductal breast carcinoma samples and corresponding normal breast tissues by using low density array PCR. Expressions of claudins (CLDNs) 5, 10, 16, 17, and 18, and ZO-1, ZO-2 were evaluated by immunohistochemistry as well. Using immunohistochemical phenotype as a surrogate for the genetic subtype, 11 luminal A, 3 luminal B, 3 triple negative and one HER2+ cases were included. Ten genes were significantly downregulated in tumors compared with normal breast tissues (CLDNs 5, 10, 16, 18, 19, CTNNAL1, JAMB, ZO-1, ZO-2 and PARD3), whereas one gene (CLDN17) was significantly up-regulated in tumors when compared with normal breast. At protein level CLDNs 5, 10, 16, 18, ZO-1 and ZO-2 were downregulated in tumors as compared with normal breast tissue. CLDN17 showed variable expression in tumor tissues in comparison to normal breast. In the single HER2+ tumor when compared with the other subtypes CLDNs 5, 16, 17, 18, CTNNAL1, JAM-B, ZO-1, ZO- 2 and PARD3 genes were found to be upregulated. We found altered TJ genes and proteins whose expression has not yet been associated with breast carcinoma. Our findings show a tendency of TJ genes and proteins to be downregulated in breast cancer. Further studies are necessary to examine whether the downregulation of the above mentioned TJ associated genes and proteins may contribute to the malignant progression of invasive ductal breast carcinomas.

Original languageEnglish
Pages (from-to)593-596
Number of pages4
JournalPathology and Oncology Research
Volume18
Issue number3
DOIs
Publication statusPublished - Jul 2012

Fingerprint

Claudins
Tight Junctions
Tight Junction Proteins
Breast
Immunohistochemistry
Breast Neoplasms
Down-Regulation
Polymerase Chain Reaction
Carcinoma, Ductal, Breast
Neoplasms
Proteins
Genes
Phenotype
Messenger RNA

Keywords

  • Array
  • Breast carcinoma
  • Claudin
  • Immunohistochemistry
  • Tight junction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Expression of tight junction molecules in breast carcinomas analysed by array PCR and immunohistochemistry. / Tőkés, A.; Szász, A.; Juhász, Éva; Schaff, Z.; Harsányi, László; Molnár, István Arthur; Baranyai, Zsolt; Besznyák, István; Zaránd, Attila; Salamon, Ferenc; Kulka, J.

In: Pathology and Oncology Research, Vol. 18, No. 3, 07.2012, p. 593-596.

Research output: Contribution to journalArticle

Tőkés, A. ; Szász, A. ; Juhász, Éva ; Schaff, Z. ; Harsányi, László ; Molnár, István Arthur ; Baranyai, Zsolt ; Besznyák, István ; Zaránd, Attila ; Salamon, Ferenc ; Kulka, J. / Expression of tight junction molecules in breast carcinomas analysed by array PCR and immunohistochemistry. In: Pathology and Oncology Research. 2012 ; Vol. 18, No. 3. pp. 593-596.
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