Expression of three isoprenoid biosynthesis genes and their effects on the carotenoid production of the zygomycete Mucor circinelloides

Árpád Csernetics, Gábor Nagy, Enrique A. Iturriaga, A. Szekeres, Arturo P. Eslava, C. Vágvölgyi, T. Papp

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The zygomycete Mucor circinelloides accumulates β-carotene as the main carotenoid compound. In this study, the applicability of some early genes of the general isoprenoid pathway to improve the carotenoid production in this fungus was examined. The isopentenyl pyrophosphate isomerase gene (ipi) was cloned and used together with the genes encoding farnesyl pyrophosphate synthase (isoA) and geranylgeranyl pyrophosphate synthase (carG) in overexpression studies. Transformation experiments showed that the first bottleneck in the pathway, from the aspect of carotenoid production, is the step controlled by the carG gene, but overexpression of the ipi and isoA genes also contributes to the availability of the precursors. Transformations with these isoprenoid genes in combination with a bacterial β-carotene ketolase gene yielded Mucor strains producing canthaxanthin and echinenone.

Original languageEnglish
Pages (from-to)696-703
Number of pages8
JournalFungal Genetics and Biology
Volume48
Issue number7
DOIs
Publication statusPublished - Jul 2011

Fingerprint

Mucor
Terpenes
Carotenoids
isopentenyldiphosphate delta-isomerase
Genes
Geranylgeranyl-Diphosphate Geranylgeranyltransferase
Canthaxanthin
Fungi

Keywords

  • β-Carotene
  • Canthaxanthin
  • FPP synthase
  • GGPP synthase
  • IPP isomerase
  • Metabolic engineering
  • Mucor

ASJC Scopus subject areas

  • Genetics
  • Microbiology

Cite this

Expression of three isoprenoid biosynthesis genes and their effects on the carotenoid production of the zygomycete Mucor circinelloides. / Csernetics, Árpád; Nagy, Gábor; Iturriaga, Enrique A.; Szekeres, A.; Eslava, Arturo P.; Vágvölgyi, C.; Papp, T.

In: Fungal Genetics and Biology, Vol. 48, No. 7, 07.2011, p. 696-703.

Research output: Contribution to journalArticle

@article{9a51771a55c842a88f3fe8aea3687a78,
title = "Expression of three isoprenoid biosynthesis genes and their effects on the carotenoid production of the zygomycete Mucor circinelloides",
abstract = "The zygomycete Mucor circinelloides accumulates β-carotene as the main carotenoid compound. In this study, the applicability of some early genes of the general isoprenoid pathway to improve the carotenoid production in this fungus was examined. The isopentenyl pyrophosphate isomerase gene (ipi) was cloned and used together with the genes encoding farnesyl pyrophosphate synthase (isoA) and geranylgeranyl pyrophosphate synthase (carG) in overexpression studies. Transformation experiments showed that the first bottleneck in the pathway, from the aspect of carotenoid production, is the step controlled by the carG gene, but overexpression of the ipi and isoA genes also contributes to the availability of the precursors. Transformations with these isoprenoid genes in combination with a bacterial β-carotene ketolase gene yielded Mucor strains producing canthaxanthin and echinenone.",
keywords = "β-Carotene, Canthaxanthin, FPP synthase, GGPP synthase, IPP isomerase, Metabolic engineering, Mucor",
author = "{\'A}rp{\'a}d Csernetics and G{\'a}bor Nagy and Iturriaga, {Enrique A.} and A. Szekeres and Eslava, {Arturo P.} and C. V{\'a}gv{\"o}lgyi and T. Papp",
year = "2011",
month = "7",
doi = "10.1016/j.fgb.2011.03.006",
language = "English",
volume = "48",
pages = "696--703",
journal = "Fungal Genetics and Biology",
issn = "1087-1845",
publisher = "Academic Press Inc.",
number = "7",

}

TY - JOUR

T1 - Expression of three isoprenoid biosynthesis genes and their effects on the carotenoid production of the zygomycete Mucor circinelloides

AU - Csernetics, Árpád

AU - Nagy, Gábor

AU - Iturriaga, Enrique A.

AU - Szekeres, A.

AU - Eslava, Arturo P.

AU - Vágvölgyi, C.

AU - Papp, T.

PY - 2011/7

Y1 - 2011/7

N2 - The zygomycete Mucor circinelloides accumulates β-carotene as the main carotenoid compound. In this study, the applicability of some early genes of the general isoprenoid pathway to improve the carotenoid production in this fungus was examined. The isopentenyl pyrophosphate isomerase gene (ipi) was cloned and used together with the genes encoding farnesyl pyrophosphate synthase (isoA) and geranylgeranyl pyrophosphate synthase (carG) in overexpression studies. Transformation experiments showed that the first bottleneck in the pathway, from the aspect of carotenoid production, is the step controlled by the carG gene, but overexpression of the ipi and isoA genes also contributes to the availability of the precursors. Transformations with these isoprenoid genes in combination with a bacterial β-carotene ketolase gene yielded Mucor strains producing canthaxanthin and echinenone.

AB - The zygomycete Mucor circinelloides accumulates β-carotene as the main carotenoid compound. In this study, the applicability of some early genes of the general isoprenoid pathway to improve the carotenoid production in this fungus was examined. The isopentenyl pyrophosphate isomerase gene (ipi) was cloned and used together with the genes encoding farnesyl pyrophosphate synthase (isoA) and geranylgeranyl pyrophosphate synthase (carG) in overexpression studies. Transformation experiments showed that the first bottleneck in the pathway, from the aspect of carotenoid production, is the step controlled by the carG gene, but overexpression of the ipi and isoA genes also contributes to the availability of the precursors. Transformations with these isoprenoid genes in combination with a bacterial β-carotene ketolase gene yielded Mucor strains producing canthaxanthin and echinenone.

KW - β-Carotene

KW - Canthaxanthin

KW - FPP synthase

KW - GGPP synthase

KW - IPP isomerase

KW - Metabolic engineering

KW - Mucor

UR - http://www.scopus.com/inward/record.url?scp=79956122715&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79956122715&partnerID=8YFLogxK

U2 - 10.1016/j.fgb.2011.03.006

DO - 10.1016/j.fgb.2011.03.006

M3 - Article

VL - 48

SP - 696

EP - 703

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

SN - 1087-1845

IS - 7

ER -