Expression of the somatostatin receptor subtype 4 in intact and inflamed pulmonary tissues

Zoltán Varecza, Krisztián Elekes, Terézia László, Anikó Perkecz, Erika Pintér, Zoltán Sándor, János Szolcsányi, Dániel Keszthelyi, Árpád Szabó, Katalin Sándor, Tamás F. Molnár, Zalán Szántó, Judit E. Pongrácz, Zsuzsanna Helyes

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Somatostatin released from capsaicin-sensitive sensory nerves of the lung during endotoxin-induced murine pneumonitis inhibits inflammation and hyperresponsiveness, presumably via somatostatin receptor subtype 4 (sst 4). The goal of the present study was to identify sst4 receptors in mouse and human lungs and to reveal its inflammation-induced alterations with real-time quantitative PCR, Western blot, and immunohistochemistry. In non-inflamed mouse and human lungs, mRNA expression and immunolocalization of sst4 are very similar. They are present on bronchial epithelial, vascular endothelial, and smooth-muscle cells. The sst4 receptor protein in the mouse lung significantly increases 24 hr after intranasal endotoxin administration as well as in response to 3 months of whole-body cigarette smoke exposure, owing to the infiltrating sst 4-positivite mononuclear cells and neutrophils. In the chronically inflamed human lung, the large number of activated macrophages markedly elevate sst4 mRNA levels, although there is no change in acute purulent pneumonia, in which granulocytes accumulate. Despite mouse granulocytes, human neutrophils do not show sst4 immunopositivity. We provide the first evidence for the expression, localization, and inflammation-induced alterations of sst4 receptors in murine and human lungs. Inasmuch as tissue distribution of this receptor is highly similar, extrapolation of murine experimental results to human conditions might be possible.

Original languageEnglish
Pages (from-to)1127-1137
Number of pages11
JournalJournal of Histochemistry and Cytochemistry
Volume57
Issue number12
DOIs
Publication statusPublished - Dec 1 2009

Keywords

  • Airway inflammation
  • Endotoxin exposure
  • Immunohistochemistry
  • Mouse and human lung pathology
  • Pulmonary macrophage
  • Reverse transcriptase polymerase chain reaction
  • Somatostatin
  • Western blot

ASJC Scopus subject areas

  • Anatomy
  • Histology

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