Expression of the carG gene, encoding geranylgeranyl pyrophosphate synthase, is up-regulated by blue light in Mucor circinelloides

Antonio Velayos, Tamás Papp, Raúl Aguilar-Elena, Mónica Fuentes-Vicente, Arturo P. Eslava, Enrique A. Iturriaga, María Isabel Álvarez

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Abstract

A new structural gene, carG, involved in the biosynthesis of carotenoids in the fungus Mucor circinelloides was isolated by heterologous hybridisation, using a probe derived from the Gibberella fujikuroi ggs1 gene. Functional analyses in Escherichia coli showed that the encoded protein has geranylgeranyl pyrophosphate (GGPP) synthase activity. A comparison of the deduced protein with other GGPP synthases suggested that the carG gene might have evolved from other larger genes present in some fungi. The analysis of carG mRNA accumulation after blue light irradiation showed that the expression of this gene is up-regulated by blue light, as happens with the other structural genes involved in carotenogenesis in M. circinelloides. Analysis of the promoter region revealed the presence of several APE-like sequences, which participate in the blue-light regulation of the expression of different fungal genes. These sequences are also present in the above-mentioned Mucor genes and strongly support the idea that this gene plays an important role in the regulation of carotenoid synthesis, despite belonging to a more general metabolic pathway.

Original languageEnglish
Pages (from-to)112-120
Number of pages9
JournalCurrent Genetics
Volume43
Issue number2
Publication statusPublished - May 1 2003

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Keywords

  • Blue-light regulation
  • Carotenoid biosynthesis
  • GGPP synthase
  • Prenyl pyrophosphate synthases

ASJC Scopus subject areas

  • Genetics

Cite this

Velayos, A., Papp, T., Aguilar-Elena, R., Fuentes-Vicente, M., Eslava, A. P., Iturriaga, E. A., & Álvarez, M. I. (2003). Expression of the carG gene, encoding geranylgeranyl pyrophosphate synthase, is up-regulated by blue light in Mucor circinelloides. Current Genetics, 43(2), 112-120.