Expression of protein phosphatase 1 during the asexual development of Neurospora crassa

Tamás Zeke, Endre Kókai, Balázs Szöor, Einat Yatzkan, Oded Yarden, Krisztina Szirák, Zsigmond Fehér, Péter Bagossi, Pál Gergely, Viktor Dombrádi

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5 Citations (Scopus)

Abstract

We cloned and sequenced the cDNA and the gene encoding the catalytic subunit of protein phosphatase 1 from the filamentous fungus Neurospora crassa. The gene, designated ppp-1 (phosphoprotein phosphatase 1), was mapped by restriction fragment length polymorphism to linkage group III, in the vicinity of con-7 and trp-1. The expression of the gene was monitored by reverse transcriptase and polymerase chain reactions, by Western blotting, and by protein phosphatase activity assays in synchronized cultures. Transcripts of ppp-1 were detected in the dormant conidia. The abundance of ppp-1 mRNA, Ppp-1 protein, and the activity of protein phosphatase 1 increased during germination and subsequent hyphal elongation as well as during the early stages of aerial mycelium formation.

Original languageEnglish
Pages (from-to)161-170
Number of pages10
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume134
Issue number1
DOIs
Publication statusPublished - Jan 1 2003

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Keywords

  • Developmental analysis
  • Gene cloning
  • Gene localization
  • Neurospora crassa
  • Phosphoprotein phosphatase 1
  • Protein dephosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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