Expression of plasma membrane ca2+-pump epitopes in human platelets and megakaryoblasts

H. Y. Cheng, M. Magocsi, R. S. Cooper, J. T. Penniston, J. L. Borke

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6 Citations (Scopus)

Abstract

Human megakaryoblastic cells (platelet precursor cells) grown in culture were sectioned for immunohistochemistry. Incubating these cells with a monoclonal antibody (JA8) directed against the human erythrocyte Ca2+ pump showed specific staining of the plasma membranes. PCR analysis of RNA from these cells demonstrated specific sequences for type HPMCA1, HPMCA2 and HPMCA4 Ca2+ pump isoforms. Western blot analysis of megakaryoblastic cells, whole platelets, and platelet membranes using monoclonal antibodies JA8, 5F10, and 3C2, directed against plasma membrane Ca2+ pump showed staining of a major band of Mr 120,000. Taken together, these studies demonstrate the existence of plasma membrane Ca2+ pump epitopes in human platelets and megakaryoblastic cells.

Original languageEnglish
Pages (from-to)31-43
Number of pages13
JournalCellular Physiology and Biochemistry
Volume4
Issue number1-2
DOIs
Publication statusPublished - Jan 1 1994

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Keywords

  • Ca pump
  • Ca-MgATPase
  • Immunohistochemistry
  • Megakaryoblast
  • PCR
  • Platelet

ASJC Scopus subject areas

  • Physiology

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