Expression of PD-L1 on immune cells shows better prognosis in laryngeal, oropharygeal, and hypopharyngeal cancer

Ede Birtalan, Kornel Danos, Bianka Gurbi, Diana Brauswetter, J. Halász, Violetta Kalocsane Piurko, Balazs Acs, Biborka Antal, Reka Mihalyi, Anna Pato, Zoltan Fent, Gabor Polony, J. Tímár, Laszlo Tamas

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Despite great enthusiasm towards immunotherapy, reliable biomarkers are still lacking. The importance of subsets based on human papillomavirus (HPV) status is supported by a growing body of evidence. However, role of other possible subgroups such as anatomic localization of primary tumor remains controversial. Our objective was to investigate immune cell infiltrate and checkpoint inhibitor proteins in above-mentioned head and neck cancer subsets. Archival tumor samples of 106 laryngeal, oropharyngeal, and hypopharyngeal cancer patients were stained with PD-L1, PD-L2, PD-1, and CTLA-4 antibodies. Proportion of tumor-infiltrating lymphocytes was assessed as well. In HPV-negative tumors, PD-L1 immune cell positivity was associated with better disease-specific survival. PD-L1 expression on immune cells correlated with improved disease-specific survival in laryngeal tumors. Furthermore, PDL1 immune cell positivity correlated with CTLA-4 expression on immune cells and it was accompanied by high tumor-infiltrating lymphocyte rate. PD-L1 expression on tumor cells and PD-1 status showed strong correlation in all patients and in oropharyngeal and laryngeal localization, but not in hypopharynx. HPVnegative oropharyngeal cancers showed negative PD-L1 status on tumor cells. CTLA-4 positivity was observed in 49.5% and 20.6% on immune cells and on tumor cells, respectively. We concluded that PD-L1 expression on immune cells indicates better prognosis in laryngeal squamous cell carcinoma and in HPV-negative head and neck squamous cell carcinoma. We have not found any essential differences between anatomic subgroups. A possibly distinct role of hypopharyngeal localization regarding immune activity requires further clarification.

Original languageEnglish
Pages (from-to)e79-e85
JournalApplied Immunohistochemistry and Molecular Morphology
Volume26
Issue number7
DOIs
Publication statusPublished - Aug 1 2018

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Hypopharyngeal Neoplasms
Laryngeal Neoplasms
Neoplasms
Oropharyngeal Neoplasms
Tumor-Infiltrating Lymphocytes
Hypopharynx
Survival
Head and Neck Neoplasms
Immunotherapy
Squamous Cell Carcinoma
Biomarkers

Keywords

  • CTLA-4
  • Head and neck cancer
  • PD-1
  • PD-L1
  • PD-L2
  • Tumor-infiltrating lymphocyte

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Medical Laboratory Technology

Cite this

Expression of PD-L1 on immune cells shows better prognosis in laryngeal, oropharygeal, and hypopharyngeal cancer. / Birtalan, Ede; Danos, Kornel; Gurbi, Bianka; Brauswetter, Diana; Halász, J.; Piurko, Violetta Kalocsane; Acs, Balazs; Antal, Biborka; Mihalyi, Reka; Pato, Anna; Fent, Zoltan; Polony, Gabor; Tímár, J.; Tamas, Laszlo.

In: Applied Immunohistochemistry and Molecular Morphology, Vol. 26, No. 7, 01.08.2018, p. e79-e85.

Research output: Contribution to journalArticle

Birtalan, E, Danos, K, Gurbi, B, Brauswetter, D, Halász, J, Piurko, VK, Acs, B, Antal, B, Mihalyi, R, Pato, A, Fent, Z, Polony, G, Tímár, J & Tamas, L 2018, 'Expression of PD-L1 on immune cells shows better prognosis in laryngeal, oropharygeal, and hypopharyngeal cancer', Applied Immunohistochemistry and Molecular Morphology, vol. 26, no. 7, pp. e79-e85. https://doi.org/10.1097/PAI.0000000000000590
Birtalan, Ede ; Danos, Kornel ; Gurbi, Bianka ; Brauswetter, Diana ; Halász, J. ; Piurko, Violetta Kalocsane ; Acs, Balazs ; Antal, Biborka ; Mihalyi, Reka ; Pato, Anna ; Fent, Zoltan ; Polony, Gabor ; Tímár, J. ; Tamas, Laszlo. / Expression of PD-L1 on immune cells shows better prognosis in laryngeal, oropharygeal, and hypopharyngeal cancer. In: Applied Immunohistochemistry and Molecular Morphology. 2018 ; Vol. 26, No. 7. pp. e79-e85.
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