Expression of p73 and Reelin in the Developing Human Cortex

Gundela Meyer, Carlos Gustavo Perez-Garcia, H. Ábrahám, Daniel Caput

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

Cajal-Retzius (CR) cells of the developing neocortex secrete Reelin (Reln), a glycoprotein involved in neuronal migration. CR cells selectively express p73, a p53 family member implicated in cell survival and apoptosis. Immunocytochemistry in prenatal human telencephalon reveals a complex sequence of migration waves of p73- and Reln-immunoreactive (IR) neurons into the cortical marginal zone (MZ). At early preplate stages, p73/Reln-IR cells arise in distinct sectors of the telencephalon, including cortical primordium and ganglionic eminences. After the appearance of the cortical plate, further p73/Reln-IR cells originate in the medial periolfactory forebrain. In addition, p73 marks a novel cell population that appears at the choroid-cortical junction or cortical hem before the emergence of the dorsal hippocampus. A pronounced mediolateral gradient in the density of p73/Reln-IR neurons in the neocortical MZ at 8 gestational weeks suggests that a subset of CR cells migrate tangentially from cortical hem and taenia tecta into neocortical territory. This hypothesis is supported by the absence of p73-transcripts in prospective neocortex of p73-/-mice at embryonic day 12 (E12), whereas they are present in cortical hem and taenia tecta. In the p73-/- preplate, Reln is faintly expressed in a calretinin-positive cell population, not present in this form in the E12 wild-type cortex. At P2, Reln-IR CR cells are undetectable in the p73-/- cortex, whereas Reln-expression in interneurons is unchanged. Our results point to a close association between p73 and Reln in CR cells of the developing neocortex, with a partial dissociation in early preplate and basal telencephalon, and to a p73-mediated role of the cortical hem in neocortical development.

Original languageEnglish
Pages (from-to)4973-4986
Number of pages14
JournalJournal of Neuroscience
Volume22
Issue number12
Publication statusPublished - Jun 15 2002

Fingerprint

Telencephalon
Neocortex
Taenia
Calbindin 2
Neurons
Choroid
Interneurons
Prosencephalon
Cerebral Cortex
Population
Hippocampus
Cell Survival
Glycoproteins
Immunohistochemistry
Apoptosis

Keywords

  • Basal telencephalon
  • Cajal-Retzius cells
  • Cortical hem
  • p73-/- mutant
  • Reelin
  • Taenia tecta

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Meyer, G., Perez-Garcia, C. G., Ábrahám, H., & Caput, D. (2002). Expression of p73 and Reelin in the Developing Human Cortex. Journal of Neuroscience, 22(12), 4973-4986.

Expression of p73 and Reelin in the Developing Human Cortex. / Meyer, Gundela; Perez-Garcia, Carlos Gustavo; Ábrahám, H.; Caput, Daniel.

In: Journal of Neuroscience, Vol. 22, No. 12, 15.06.2002, p. 4973-4986.

Research output: Contribution to journalArticle

Meyer, G, Perez-Garcia, CG, Ábrahám, H & Caput, D 2002, 'Expression of p73 and Reelin in the Developing Human Cortex', Journal of Neuroscience, vol. 22, no. 12, pp. 4973-4986.
Meyer G, Perez-Garcia CG, Ábrahám H, Caput D. Expression of p73 and Reelin in the Developing Human Cortex. Journal of Neuroscience. 2002 Jun 15;22(12):4973-4986.
Meyer, Gundela ; Perez-Garcia, Carlos Gustavo ; Ábrahám, H. ; Caput, Daniel. / Expression of p73 and Reelin in the Developing Human Cortex. In: Journal of Neuroscience. 2002 ; Vol. 22, No. 12. pp. 4973-4986.
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