Expression of NTPDase1 and caveolins in human cardiovascular disease

A. Kittel, A. Kiss, Nándor Müllner, Ida Matkó, B. Sperlágh

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Pathological circumstances like inflammation or ischemic insult facilitate the release of adenine nucleotides from several types of cells. These extracellular nucleotides are rapidly converted to adenosine by ectonucleotidases, mainly ectonucleoside triphosphate diphosphohydrolase1 (NTPDase1/CD39) and CD73. NTPDase1/CD39 can interact with caveolins, structural proteins of signal-transducing microdomains termed caveolae. Caveolins are thought to have physiological roles in heart ageing and cardiac diseases. The aim of this study was to investigate the expression of NTPDase1 together with caveolins in chronic human cardiovascular diseases and elucidate their role in human heart. The HPLC analysis showed significant increase in ATPase activity in pathological samples from patients with ischemic heart disease. Immunostaining also showed alterations in the expression and distribution of NTPDase1. Caveolin-1 and caveolin-2 expression was much alike in control and pathological cases, while expression of caveolin-3 was lower in pathological samples. Changes in the expression of NTPDase1 and caveolins seem to be independent of human cardiovascular disease.

Original languageEnglish
Pages (from-to)51-59
Number of pages9
JournalHistochemistry and Cell Biology
Volume124
Issue number1
DOIs
Publication statusPublished - Aug 2005

Fingerprint

Caveolins
Cardiovascular Diseases
Nucleotides
nucleotides
Heart Diseases
Caveolin 2
Caveolin 3
heart diseases
Caveolin 1
Caveolae
adenosines
Adenine Nucleotides
adenines
Aging of materials
Adenosine
Myocardial Ischemia
Adenosine Triphosphatases
Proteins
High Pressure Liquid Chromatography
CD39 antigen

Keywords

  • Caveolins
  • Enzyme histochemistry
  • HPLC
  • NTPDase1
  • Ultracryo immunohistochemistry

ASJC Scopus subject areas

  • Cell Biology
  • Instrumentation

Cite this

Expression of NTPDase1 and caveolins in human cardiovascular disease. / Kittel, A.; Kiss, A.; Müllner, Nándor; Matkó, Ida; Sperlágh, B.

In: Histochemistry and Cell Biology, Vol. 124, No. 1, 08.2005, p. 51-59.

Research output: Contribution to journalArticle

@article{b9984e7ce4bc4398922cf960e0e9b19c,
title = "Expression of NTPDase1 and caveolins in human cardiovascular disease",
abstract = "Pathological circumstances like inflammation or ischemic insult facilitate the release of adenine nucleotides from several types of cells. These extracellular nucleotides are rapidly converted to adenosine by ectonucleotidases, mainly ectonucleoside triphosphate diphosphohydrolase1 (NTPDase1/CD39) and CD73. NTPDase1/CD39 can interact with caveolins, structural proteins of signal-transducing microdomains termed caveolae. Caveolins are thought to have physiological roles in heart ageing and cardiac diseases. The aim of this study was to investigate the expression of NTPDase1 together with caveolins in chronic human cardiovascular diseases and elucidate their role in human heart. The HPLC analysis showed significant increase in ATPase activity in pathological samples from patients with ischemic heart disease. Immunostaining also showed alterations in the expression and distribution of NTPDase1. Caveolin-1 and caveolin-2 expression was much alike in control and pathological cases, while expression of caveolin-3 was lower in pathological samples. Changes in the expression of NTPDase1 and caveolins seem to be independent of human cardiovascular disease.",
keywords = "Caveolins, Enzyme histochemistry, HPLC, NTPDase1, Ultracryo immunohistochemistry",
author = "A. Kittel and A. Kiss and N{\'a}ndor M{\"u}llner and Ida Matk{\'o} and B. Sperl{\'a}gh",
year = "2005",
month = "8",
doi = "10.1007/s00418-005-0018-8",
language = "English",
volume = "124",
pages = "51--59",
journal = "Histochemie. Histochemistry. Histochimie",
issn = "0948-6143",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Expression of NTPDase1 and caveolins in human cardiovascular disease

AU - Kittel, A.

AU - Kiss, A.

AU - Müllner, Nándor

AU - Matkó, Ida

AU - Sperlágh, B.

PY - 2005/8

Y1 - 2005/8

N2 - Pathological circumstances like inflammation or ischemic insult facilitate the release of adenine nucleotides from several types of cells. These extracellular nucleotides are rapidly converted to adenosine by ectonucleotidases, mainly ectonucleoside triphosphate diphosphohydrolase1 (NTPDase1/CD39) and CD73. NTPDase1/CD39 can interact with caveolins, structural proteins of signal-transducing microdomains termed caveolae. Caveolins are thought to have physiological roles in heart ageing and cardiac diseases. The aim of this study was to investigate the expression of NTPDase1 together with caveolins in chronic human cardiovascular diseases and elucidate their role in human heart. The HPLC analysis showed significant increase in ATPase activity in pathological samples from patients with ischemic heart disease. Immunostaining also showed alterations in the expression and distribution of NTPDase1. Caveolin-1 and caveolin-2 expression was much alike in control and pathological cases, while expression of caveolin-3 was lower in pathological samples. Changes in the expression of NTPDase1 and caveolins seem to be independent of human cardiovascular disease.

AB - Pathological circumstances like inflammation or ischemic insult facilitate the release of adenine nucleotides from several types of cells. These extracellular nucleotides are rapidly converted to adenosine by ectonucleotidases, mainly ectonucleoside triphosphate diphosphohydrolase1 (NTPDase1/CD39) and CD73. NTPDase1/CD39 can interact with caveolins, structural proteins of signal-transducing microdomains termed caveolae. Caveolins are thought to have physiological roles in heart ageing and cardiac diseases. The aim of this study was to investigate the expression of NTPDase1 together with caveolins in chronic human cardiovascular diseases and elucidate their role in human heart. The HPLC analysis showed significant increase in ATPase activity in pathological samples from patients with ischemic heart disease. Immunostaining also showed alterations in the expression and distribution of NTPDase1. Caveolin-1 and caveolin-2 expression was much alike in control and pathological cases, while expression of caveolin-3 was lower in pathological samples. Changes in the expression of NTPDase1 and caveolins seem to be independent of human cardiovascular disease.

KW - Caveolins

KW - Enzyme histochemistry

KW - HPLC

KW - NTPDase1

KW - Ultracryo immunohistochemistry

UR - http://www.scopus.com/inward/record.url?scp=24344499868&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24344499868&partnerID=8YFLogxK

U2 - 10.1007/s00418-005-0018-8

DO - 10.1007/s00418-005-0018-8

M3 - Article

C2 - 16028070

AN - SCOPUS:24344499868

VL - 124

SP - 51

EP - 59

JO - Histochemie. Histochemistry. Histochimie

JF - Histochemie. Histochemistry. Histochimie

SN - 0948-6143

IS - 1

ER -