Expression of mRNA for human type-I LHRH receptor transcript forms in human benign prostatic hyperplasia

Bernadett Rózsa, Aliz Juhász, Andrea Treszl, György Tóth, Tibor Flaskó, Balázs Dezsö, Norman L. Block, Andrew V. Schally, Gábor Halmos

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3 Citations (Scopus)

Abstract

The presence of four different isoforms of luteinizing hormone-releasing hormones (LHRH) and one LHRH receptor (LHRH-R) has been reported in vertebrates. In the human genome only LHRH-I and LHRH-II genes have been identified. The human LHRH-I gene is composed of four exons separated by three introns. Three LHRH receptor or receptor-like genes have been demonstrated. The well-established type-I LHRH receptor (LHRH-R-I) gene is composed of three exons separated by two introns. In this study we investigated the expression of transcript forms of LHRH-R-I in human benign prostatic hyperplasia (BPH) with reverse transcriptase-polymerase chain reaction (RT-PCR) using gene specific primers. Thirty-five human BPH specimens were obtained at surgery. Normal human pituitaries collected at autopsy served as control. RNA extraction and RT-PCR with gene-specific primers for LHRH-R-I forward (F1)/reverse (R1), LHRH-R-I F2/R3, LHRH-R-I F1′/R2′ were carried out to determine the mRNA expression for LHRH-R-I transcript forms. The expected PCR products amplified with gene specific primers were LHRH-R-I F1/R1 with 319 bp, LHRHR-I F2/R3 with 309 bp and LHRH-R-I F1′/R2′ with 219 bp. PCR products for LHRH-R-I F1/R1 were detected in 21 (60%) and for LHRH-R-I F2/R3 in 5 of 35 (14%) BPH samples. No PCR products for LHRH-R-I F1′/R2′ were found. In conclusion, we detected mRNA for LHRH-R-I in human BPH specimens. Our results suggest that LHRH-R-I gene may have more than two splice variants or uncharacterised transcript forms of LHRH-R-I. Our findings support the merit of further investigation of the expression of LHRH-R-I and its transcript forms in human BPH.

Original languageEnglish
Pages (from-to)1053-1059
Number of pages7
JournalInternational journal of oncology
Volume35
Issue number5
DOIs
Publication statusPublished - Nov 1 2009

Keywords

  • Human benign prostatic hyperplasia
  • Receptors for LHRH
  • Transcript forms

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Rózsa, B., Juhász, A., Treszl, A., Tóth, G., Flaskó, T., Dezsö, B., Block, N. L., Schally, A. V., & Halmos, G. (2009). Expression of mRNA for human type-I LHRH receptor transcript forms in human benign prostatic hyperplasia. International journal of oncology, 35(5), 1053-1059. https://doi.org/10.3892/ijo-00000420