Expression of membrane-bound and soluble cell adhesion molecules by human brain microvessel endothelial cells

Monika Vastag, Judit Skopál, Zoltán Voko, Éva Csonka, Zoltán Nagy

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Expression of membrane-bound (mb) and soluble (s) forms of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) induced by tumor necrosis factor-α (TNF-α) has been measured by enzyme- linked immunosorbent assay in cultured human brain microvessel endothelial cells. Both the mb and the s forms of VCAM-1 and ICAM-1 were upregulated by TNF-α; however, the stimulation of the s forms was delayed in time. When piracetam, a neuroprotective drug, was added to the tissue culture medium simultaneously with TNF-α, the expression of mbVCAM-1 and ICAM-1 was lowered. Differential upregulation of mb and s forms of adhesion molecules and a novel effect of piracetam have been demonstrated in human brain microvessel endothelial cell cultures.

Original languageEnglish
Pages (from-to)52-60
Number of pages9
JournalMicrovascular Research
Volume57
Issue number1
DOIs
Publication statusPublished - Jan 1999

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Keywords

  • Adhesion molecules
  • Human brain microvessel endothelium
  • Piracetam
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

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