Expression of membrane-bound and soluble cell adhesion molecules by human brain microvessel endothelial cells

Monika Vastag, Judit Skopál, Z. Vokó, Éva Csonka, Zoltán Nagy

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Expression of membrane-bound (mb) and soluble (s) forms of vascular cell adhesion molecule-1 (VCAM-1) and intercellular adhesion molecule-1 (ICAM-1) induced by tumor necrosis factor-α (TNF-α) has been measured by enzyme- linked immunosorbent assay in cultured human brain microvessel endothelial cells. Both the mb and the s forms of VCAM-1 and ICAM-1 were upregulated by TNF-α; however, the stimulation of the s forms was delayed in time. When piracetam, a neuroprotective drug, was added to the tissue culture medium simultaneously with TNF-α, the expression of mbVCAM-1 and ICAM-1 was lowered. Differential upregulation of mb and s forms of adhesion molecules and a novel effect of piracetam have been demonstrated in human brain microvessel endothelial cell cultures.

Original languageEnglish
Pages (from-to)52-60
Number of pages9
JournalMicrovascular Research
Volume57
Issue number1
DOIs
Publication statusPublished - Jan 1999

Fingerprint

Endothelial cells
Cell Adhesion Molecules
Intercellular Adhesion Molecule-1
Microvessels
Piracetam
Brain
Endothelial Cells
Vascular Cell Adhesion Molecule-1
Tumor Necrosis Factor-alpha
Membranes
Tissue culture
Immunosorbents
Neuroprotective Agents
Cell culture
Culture Media
Assays
Up-Regulation
Adhesion
Cell Culture Techniques
Enzyme-Linked Immunosorbent Assay

Keywords

  • Adhesion molecules
  • Human brain microvessel endothelium
  • Piracetam
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine

Cite this

Expression of membrane-bound and soluble cell adhesion molecules by human brain microvessel endothelial cells. / Vastag, Monika; Skopál, Judit; Vokó, Z.; Csonka, Éva; Nagy, Zoltán.

In: Microvascular Research, Vol. 57, No. 1, 01.1999, p. 52-60.

Research output: Contribution to journalArticle

Vastag, Monika ; Skopál, Judit ; Vokó, Z. ; Csonka, Éva ; Nagy, Zoltán. / Expression of membrane-bound and soluble cell adhesion molecules by human brain microvessel endothelial cells. In: Microvascular Research. 1999 ; Vol. 57, No. 1. pp. 52-60.
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