Expression of latent transforming growth factor beta binding proteins in the rat brain

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Transforming growth factor-betas are expressed in the brain, have neuroprotective functions, and may be involved in the pathogenesis of neurodegenerative disorders. Their intracellular processing, secretion, and extracellular activation requires latent transforming growth factor-beta binding proteins (LTBPs) as demonstrated in peripheral organs. Here, we first report that the four types of LTBPs are expressed in the rat brain based on reverse-transcriptase polymerase chain reaction (RT-PCR) and that the subtypes have different topographical distributions based on in situ hybridization histochemistry. LTBP-1 has a high expression level in several brain regions including choroid plexus, cerebral cortex, medial amygdaloid nucleus, anteromedial and midline thalamic nuclei, medial preoptic area, arcuate and dorsomedial hypothalamic nuclei, superior olive, and area postrema. LTBP-3 and -4 are the most widely distributed LTBPs. Both are abundant in the cerebral cortex, cerebellum, hypothalamus, amygdala, brainstem motor nuclei, and area postrema. In addition, LTBP-3 mRNA is also abundant in the choroid plexus, globus pallidus, anterior and reticular thalamic nuclei, mamillary body, substantia nigra, red nucleus, pontine nuclei, some brainstem sensory nuclei, and reticular formation, while LTBP-4 is more abundant in the hippocampus and the parabrachial nuclei. In contrast, the expression of LTBP-2 is restricted to cerebral cortex, CA1 neurons of the hippocampus, and perifornical/lateral hypothalamic areas. The hypothalamic cells were identified by double in situ hybridization histochemistry as orexin-synthesizing neurons, demonstrating that LTBP expression can be very specifically regulated. Our data demonstrate that each type of LTBPs have highly distinct distributional patterns suggesting that the expression of LTBPs are specifically regulated in the brain.

Original languageEnglish
Pages (from-to)1393-1408
Number of pages16
JournalJournal of Comparative Neurology
Volume507
Issue number3
DOIs
Publication statusPublished - Mar 20 2008

Fingerprint

Transforming Growth Factor beta
Carrier Proteins
Cerebral Cortex
Anterior Thalamic Nuclei
Area Postrema
Choroid Plexus
Brain
Brain Stem
In Situ Hybridization
Hippocampus
Dorsomedial Hypothalamic Nucleus
Midline Thalamic Nuclei
Mammillary Bodies
Lateral Hypothalamic Area
Red Nucleus
Neurons
Globus Pallidus
Preoptic Area
Reticular Formation
Motor Cortex

Keywords

  • Brain distribution
  • In situ hybridization histochemistry
  • Mapping
  • mRNA expression
  • Neuroprotection
  • Orexin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Expression of latent transforming growth factor beta binding proteins in the rat brain. / Dobolyi, A.; Palkóvits, M.

In: Journal of Comparative Neurology, Vol. 507, No. 3, 20.03.2008, p. 1393-1408.

Research output: Contribution to journalArticle

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