Expression of HMGB-1 and its extracellular release of cultured primary hepatic parenchymal cells and Kupffer cells induced by LPS

Z. Zhao, De wu Han, Ming she Liu, Guo ying Zhang, Yun Zhang, Hui Yang, Liu xu Yang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

OBJECTIVE: To investigate HMGB-1 expression and its extracellular release of cultured primary hepatic parenchymal cells (HC) and Kupffer cells (KC) that were induced by lipopolysaccharides (LPS). METHODS: Primary hepatic parenchymal cells and Kupffer cells were cultured in flasks, and some cells were treated with 500 microg/L LPS for 24 hours (induced group) and some were not treated with LPS and served as controls. All of the cells were repeatedly frozen-thawed, and the expression levels of HMGB1-mRNA and HMGB1 proteins were detected by semi-quantitative RT-PCR and Western blot respectively. Then HC and KC were subcultured in 24-well culture plates for 6 h, 12 h, 24 h and 48 h, and the HMGB1 protein in culture fluids was detected by Western blot at each time point. RESULTS: Compared with the cells in the control group, the expression levels of HMGB1-mRNA in the induced group were significantly increased in both HC and KC at 24 h (t=31.32 and 45.90, P0.05) but was significantly increased at 12 h, 24 h and 48 h (t=21.95, 32.39, 44.16, respectively P

Original languageEnglish
Pages (from-to)676-680
Number of pages5
JournalZhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology
Volume15
Issue number9
Publication statusPublished - Sep 2007

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HMGB Proteins
HMGB1 Protein
Kupffer Cells
Lipopolysaccharides
Hepatocytes
Western Blotting
Messenger RNA
Polymerase Chain Reaction
Control Groups

ASJC Scopus subject areas

  • Medicine(all)

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Expression of HMGB-1 and its extracellular release of cultured primary hepatic parenchymal cells and Kupffer cells induced by LPS. / Zhao, Z.; Han, De wu; Liu, Ming she; Zhang, Guo ying; Zhang, Yun; Yang, Hui; Yang, Liu xu.

In: Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology, Vol. 15, No. 9, 09.2007, p. 676-680.

Research output: Contribution to journalArticle

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AU - Zhao, Z.

AU - Han, De wu

AU - Liu, Ming she

AU - Zhang, Guo ying

AU - Zhang, Yun

AU - Yang, Hui

AU - Yang, Liu xu

PY - 2007/9

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