Expression of hexose-6-phosphate dehydrogenase in rat tissues

Paola Marcolongo, Silvia Senesi, Roberta Giunti, Miklós Csala, Rosella Fulceri, Gábor Bánhegyi, Angelo Benedetti

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Hexose-6-phosphate dehydrogenase (H6PD) is the main NADPH generating enzyme in the lumen of the endoplasmic reticulum. H6PD is regarded as an ancillary enzyme in prereceptorial glucocorticoid activation and probably acts as a nutrient sensor and as a prosurvival factor. H6PD expression was determined in a variety of rat and human tissues by detecting mRNA and protein levels, and by measuring its dehydrogenase and lactonase activities. It was found that H6PD was present in all investigated tissues; both expression and activity remained within an order of magnitude. Correlation was found between the dehydrogenase activity and protein or mRNA levels. The results confirmed the supposed housekeeping feature of the enzyme.

Original languageEnglish
Pages (from-to)57-64
Number of pages8
JournalJournal of Steroid Biochemistry and Molecular Biology
Volume126
Issue number3-5
DOIs
Publication statusPublished - Sep 1 2011

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Keywords

  • 11β-Hydroxysteroid dehydrogenase type 1
  • Endoplasmic reticulum
  • Glucose-6-phosphate
  • Hexose-6-phosphate dehydrogenase
  • NADPH

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology

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