Expression of heteromeric glycine receptor-channels in rat spinal cultures and inhibition by neuroactive steroids

László Fodor, András Boros, Péter Dezso, G. Maksay

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Ionotropic glycine receptors were studied in cultured spinal cord neurons prepared from 17-day-old rat embryos, using whole-cell patch clamp electrophysiology. Glycine receptors of 3-17 days in vitro were characterized via subtype-specific channel blockade by micromolar picrotoxin and cyanotriphenylborate, as well as nanomolar strychnine. Potentiation by nanomolar tropisetron indicated coexpression of β with α subunits. The neuroactive steroids pregnenolone sulfate and dehydroepiandrosterone sulfate, as well as alphaxalone and its 3β epimer betaxalone inhibited the chloride current with IC50 values of 19, 46, 16 and 208 μM, respectively, with no potentiation. Reverse transcription polymerase chain reaction and immunocytochemistry demonstrated mRNAs and proteins of α1, α2, α3 and β subunits in rat spinal cord cultures. In conclusion, neuroactive steroids, both positive and negative modulators of γ-aminobutyric-acidA receptors, inhibited heteromeric glycine receptors at micromolar concentrations.

Original languageEnglish
Pages (from-to)577-583
Number of pages7
JournalNeurochemistry International
Volume49
Issue number6
DOIs
Publication statusPublished - Nov 2006

Fingerprint

Glycine Receptors
tropisetron
Steroids
Spinal Cord
Strychnine
Picrotoxin
Dehydroepiandrosterone Sulfate
Electrophysiology
Inhibitory Concentration 50
Reverse Transcription
Chlorides
Embryonic Structures
Immunohistochemistry
Neurons
Polymerase Chain Reaction
Messenger RNA
Proteins

Keywords

  • Alphaxalone
  • Betaxalone
  • Electrophysiology
  • Glycine receptors of spinal cord culture
  • Neurosteroid
  • Protein and mRNA expression
  • Tropisetron

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Expression of heteromeric glycine receptor-channels in rat spinal cultures and inhibition by neuroactive steroids. / Fodor, László; Boros, András; Dezso, Péter; Maksay, G.

In: Neurochemistry International, Vol. 49, No. 6, 11.2006, p. 577-583.

Research output: Contribution to journalArticle

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