Expression of hemin receptor molecule ChuA is influenced by RfaH in uropathogenic Escherichia coli strain 536

G. Nagy, U. Dobrindt, M. Kupfer, L. Emődy, H. Karch, J. Hacker

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The outer membrane protein ChuA responsible for heroin utilization has been recently identified in several pathogenic Escherichia coli strains. We report that the regulatory, protein RfaH influences ChuA expression in the uropathogenic E. coli strain 536. In an rfaH mutant, the chuA transcript as well as the ChuA protein levels were significantly decreased in comparison with those in the wild-type strain. Within the chuA gene, a consensus motif known as the JUMPStart (just upstream of many polysaccharide associated gene starts) sequence was found, which is shared by RfaH-affected operons. Furthermore, the presence of two different subclasses of the chuA determinant and their distribution in E. coli pathogroups are described.

Original languageEnglish
Pages (from-to)1924-1928
Number of pages5
JournalInfection and Immunity
Volume69
Issue number3
DOIs
Publication statusPublished - 2001

Fingerprint

Uropathogenic Escherichia coli
Hemin
Escherichia coli
Heroin
Operon
Genes
Polysaccharides
Membrane Proteins
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Expression of hemin receptor molecule ChuA is influenced by RfaH in uropathogenic Escherichia coli strain 536. / Nagy, G.; Dobrindt, U.; Kupfer, M.; Emődy, L.; Karch, H.; Hacker, J.

In: Infection and Immunity, Vol. 69, No. 3, 2001, p. 1924-1928.

Research output: Contribution to journalArticle

Nagy, G. ; Dobrindt, U. ; Kupfer, M. ; Emődy, L. ; Karch, H. ; Hacker, J. / Expression of hemin receptor molecule ChuA is influenced by RfaH in uropathogenic Escherichia coli strain 536. In: Infection and Immunity. 2001 ; Vol. 69, No. 3. pp. 1924-1928.
@article{f2b04044c7d4482c862c20165d386c8a,
title = "Expression of hemin receptor molecule ChuA is influenced by RfaH in uropathogenic Escherichia coli strain 536",
abstract = "The outer membrane protein ChuA responsible for heroin utilization has been recently identified in several pathogenic Escherichia coli strains. We report that the regulatory, protein RfaH influences ChuA expression in the uropathogenic E. coli strain 536. In an rfaH mutant, the chuA transcript as well as the ChuA protein levels were significantly decreased in comparison with those in the wild-type strain. Within the chuA gene, a consensus motif known as the JUMPStart (just upstream of many polysaccharide associated gene starts) sequence was found, which is shared by RfaH-affected operons. Furthermore, the presence of two different subclasses of the chuA determinant and their distribution in E. coli pathogroups are described.",
author = "G. Nagy and U. Dobrindt and M. Kupfer and L. Emődy and H. Karch and J. Hacker",
year = "2001",
doi = "10.1128/IAI.69.3.1924-1928.2001",
language = "English",
volume = "69",
pages = "1924--1928",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Expression of hemin receptor molecule ChuA is influenced by RfaH in uropathogenic Escherichia coli strain 536

AU - Nagy, G.

AU - Dobrindt, U.

AU - Kupfer, M.

AU - Emődy, L.

AU - Karch, H.

AU - Hacker, J.

PY - 2001

Y1 - 2001

N2 - The outer membrane protein ChuA responsible for heroin utilization has been recently identified in several pathogenic Escherichia coli strains. We report that the regulatory, protein RfaH influences ChuA expression in the uropathogenic E. coli strain 536. In an rfaH mutant, the chuA transcript as well as the ChuA protein levels were significantly decreased in comparison with those in the wild-type strain. Within the chuA gene, a consensus motif known as the JUMPStart (just upstream of many polysaccharide associated gene starts) sequence was found, which is shared by RfaH-affected operons. Furthermore, the presence of two different subclasses of the chuA determinant and their distribution in E. coli pathogroups are described.

AB - The outer membrane protein ChuA responsible for heroin utilization has been recently identified in several pathogenic Escherichia coli strains. We report that the regulatory, protein RfaH influences ChuA expression in the uropathogenic E. coli strain 536. In an rfaH mutant, the chuA transcript as well as the ChuA protein levels were significantly decreased in comparison with those in the wild-type strain. Within the chuA gene, a consensus motif known as the JUMPStart (just upstream of many polysaccharide associated gene starts) sequence was found, which is shared by RfaH-affected operons. Furthermore, the presence of two different subclasses of the chuA determinant and their distribution in E. coli pathogroups are described.

UR - http://www.scopus.com/inward/record.url?scp=0035112106&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035112106&partnerID=8YFLogxK

U2 - 10.1128/IAI.69.3.1924-1928.2001

DO - 10.1128/IAI.69.3.1924-1928.2001

M3 - Article

C2 - 11179376

AN - SCOPUS:0035112106

VL - 69

SP - 1924

EP - 1928

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 3

ER -