Expression of guanylin in 'pars tuberalis-specific cells' and gonadotrophs of rat adenohypophysis

Loredana D'Este, Hasan Kulaksiz, Ulrich Rausch, Rosa Vaccaro, T. Wenger, Yoshimitsu Tokunaga, Tindaro G. Renda, Yalcin Cetin

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The intestinal peptide guanylin regulates the electrolyte/water transport in the gastrointestinal epithelium by paracrine/luminocrine mechanisms. Because guanylin also circulates in the blood, we investigated the rat hypothalamo-pituitary region for expression and cellular localization of this peptide. Reverse transcriptase-PCR analyses with guanylin-specific primers revealed expression of the peptide in the pars tuberalis and pars distalis of the pituitary. Western blotting analyses in hypophyseal tissue extracts identified the expected 12.5-kDa immunoreactive peptide by using two different region-specific guanylin antisera. Light and electron microscopic immunocytochemistry with the same antisera localized guanylin in 'pars tuberalis-specific cells' in the juxtaneural pars tuberalis adjacent to nerve endings and blood vessels of the hypothalamo-pituitary portal system and in gonadotrophic cells within the distal pars tuberalis and ventrolateral part of the pars distalis. The presence and cell-specific localization of guanylin within the hypothalamo-hypophyseal system indicate that this peptide may be specifically involved in paracrine and endocrine regulatory mechanisms.

Original languageEnglish
Pages (from-to)1131-1136
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number3
DOIs
Publication statusPublished - 2000

Fingerprint

Gonadotrophs
Anterior Pituitary Gland
Peptides
Immune Sera
Hypothalamo-Hypophyseal System
Portal System
Tissue Extracts
Nerve Endings
Reverse Transcriptase Polymerase Chain Reaction
Electrolytes
Blood Vessels
guanylin
Epithelium
Western Blotting
Immunohistochemistry
Electrons
Light
Water

Keywords

  • Gonadotrophic cells
  • Guanylate cyclase C-activating peptides
  • Pituitary gland
  • Regulatory peptides

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Expression of guanylin in 'pars tuberalis-specific cells' and gonadotrophs of rat adenohypophysis. / D'Este, Loredana; Kulaksiz, Hasan; Rausch, Ulrich; Vaccaro, Rosa; Wenger, T.; Tokunaga, Yoshimitsu; Renda, Tindaro G.; Cetin, Yalcin.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 3, 2000, p. 1131-1136.

Research output: Contribution to journalArticle

D'Este, Loredana ; Kulaksiz, Hasan ; Rausch, Ulrich ; Vaccaro, Rosa ; Wenger, T. ; Tokunaga, Yoshimitsu ; Renda, Tindaro G. ; Cetin, Yalcin. / Expression of guanylin in 'pars tuberalis-specific cells' and gonadotrophs of rat adenohypophysis. In: Proceedings of the National Academy of Sciences of the United States of America. 2000 ; Vol. 97, No. 3. pp. 1131-1136.
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