Expression of genes related to oxidative/nitrosative stress in mouse hearts: Effect of preconditioning and cholesterol diet

Gabriella F. Kocsis, T. Csont, Zoltán Varga-Orvos, L. Puskás, Zsolt Murlasits, P. Ferdinándy

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: The aim of our study was to explore the effect of high-cholesterol diet and preconditioning on cardiac gene expression patterns in mouse hearts, focusing on genes involved in nitric oxide (NO) and free radical signaling and the mevalonate pathway. Material/Methods: Mice were fed 2% high-cholesterol or normal diet for 8 weeks. Hearts isolated from both groups were subjected to either a preconditioning (PC) protocol (3 cycles of 5 min ischemia and 5 min aerobic perfusion) or a time-matched non-preconditioning protocol followed by 30 min global test ischemia and 2 hour reperfusion. Results: PC altered gene expression only in the mice subjected to a normal diet, as shown in neuronal nitric oxide synthase (nNOS), endothelial nitric oxide synthase (eNOS) and the superoxide-producing enzymes xanthine oxidase (XO) and nicotinamide adenine dinucleotide phosphate (NADPH) oxidases 1 and 4. The rate-limiting enzyme of the mevalonate pathway, 3-Hydroxy-3-methylglutaryl- coenzyme A (HMG-CoA) reductase showed differential expression in the myocardium in response to I/R and PC in mice on normal diet but not in cholesterol-fed animals. Conclusions: We conclude that cholesterol-enriched diet leads to alterations in preconditioning-induced gene expression in the mouse heart, which might lead to marked changes of oxidative/nitrosative stress signaling and to the attenuation of the cardioprotective effect of preconditioning.

Original languageEnglish
JournalMedical Science Monitor
Volume16
Issue number1
Publication statusPublished - Jan 2010

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Oxidative Stress
Cholesterol
Diet
Gene Expression
Mevalonic Acid
Oxidoreductases
Ischemia
Nitric Oxide Synthase Type I
Xanthine Oxidase
Nitric Oxide Synthase Type III
Enzymes
NADP
Superoxides
Reperfusion
Free Radicals
Myocardium
Nitric Oxide
Perfusion
Genes

Keywords

  • Cardioprotection
  • Hypercholesterolemia
  • Ischemia-reperfusion
  • Preconditioning

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Expression of genes related to oxidative/nitrosative stress in mouse hearts : Effect of preconditioning and cholesterol diet. / Kocsis, Gabriella F.; Csont, T.; Varga-Orvos, Zoltán; Puskás, L.; Murlasits, Zsolt; Ferdinándy, P.

In: Medical Science Monitor, Vol. 16, No. 1, 01.2010.

Research output: Contribution to journalArticle

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