Expression of FMRFamide gene encoded peptides by identified neurons in embryos and juveniles of the pulmonate snail Lymnaea stagnalis

E. E. Voronezhskaya, K. Elekes

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Immunoreactivity to antibodies (ABs) against FMRFamide, CARP, and three FMRFamide gene encoded peptides, i.e., EFLRIamide, the non-FMRFamide peptide "SEEPLY," and the 35-amino-acid "acidic peptide," were investigated in developing embryos and juveniles of Lymnaea stagnalis. Five early transient embryonic neurons revealed immunoreactivity to EFLRIamide. One of the early neurons, the central posterior, also expressed SEEPLY and CARP immunoreactivity. Two neurons in the anlage of the left and right parietal ganglia coexpressed immunoreactivity to EFLRIamide (type 1 transcript) and acidic peptide (type 2 transcript). Within the developing ganglia altogether 30 neurons expressed the type 1 transcript, and three expressed the type 2 transcript. No peripheral cells immunoreactive to SEEPLY or acidic peptide ABs were found, whereas bipolar EFLRIamide- and CARP-immunoreactive cells were abundant in the lip, mantle and foot. After hatching, the number of immunoreactive neurons in ganglia increased up to 223 and the neurons expressing tetrapeptides were dominant (91%). No neurons coexpressing type 1 transcript and type 2 transcript could be detected in juveniles and adults. At this time, an extensive innervation is developed in the periphery, including foot, mantle, buccal mass, salivary glands and alimentary tract, established mainly by EFLRIamide-immunoreactive cells and varicose fibers of extrinsic and intrinsic origin. It is suggested that both sensory and regulatory function can be attributed to the FMRFamide gene encoded tetrapeptides throughout embryonic and juvenile development in Lymnaea, whereas heptapeptides are presumed to play a modulatory role.

Original languageEnglish
Pages (from-to)297-313
Number of pages17
JournalCell And Tissue Research
Volume314
Issue number2
DOIs
Publication statusPublished - Nov 1 2003

Keywords

  • Central and peripheral nervous system
  • Embryogenesis
  • Immunocytochemistry
  • Lymnaea stagnalis (Mollusca)
  • Neuronal development
  • Neuropeptides

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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