Expression of extracellular matrix proteoglycans perlecan and decorin in carbon-tetrachloride-injured rat liver and in isolated liver cells

M. Gallai, I. Kovalszky, Thomas Knittel, Katrin Neubauer, Thomas Armbrust, Giuliano Ramadori

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Abstract

Proteoglycansare important components of the extracellular matrix. They are involved in liver regeneration as well as in liver fibrosis. The distribution and cellular source of proteoglycans under normal as well as pathological conditions is still under debate. Localization of decorin and perlecan was studied in normal, acutely damaged, and cirrhotic liver by histochemistry. Furthermore, their synthesis was analyzed in different liver cell populations isolated from normal rat liver. In normal liver, decorin positivity was observed in the perisinusoidal space and in the portal area. Perlecan was clearly detectable in the portal area (blood vessels and bile ducts); a moderate reaction was also seen along the sinusoids. Strong positivity for both proteoglycans was detectable in the necrotic areas of acutely damaged liver. Chronic liver damage was characterized by the deposition of decorin and perlecan in the fibrotic septa. Immunocytochemical reactions were positive for perlecan and decorin in cultured Ito and endothelial cells but not in hepatocytes and Kupffer cells. Northern hybridization confirmed the capacity of Ito cells and endothelial cells to express the two genes. Interestingly, although rat skin fibroblasts expressed strong messages for both proteoglycans, rat aortic smooth muscle cells did not synthesize decorin.

Original languageEnglish
Pages (from-to)1463-1471
Number of pages9
JournalAmerican Journal of Pathology
Volume148
Issue number5
Publication statusPublished - May 1996

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Decorin
Carbon Tetrachloride
Proteoglycans
Extracellular Matrix
Liver
Hepatic Stellate Cells
Endothelial Cells
Liver Regeneration
Kupffer Cells
Bile Ducts
Liver Cirrhosis
Smooth Muscle Myocytes
Blood Vessels
perlecan
Hepatocytes
Cultured Cells
Fibroblasts
Skin
Population
Genes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Expression of extracellular matrix proteoglycans perlecan and decorin in carbon-tetrachloride-injured rat liver and in isolated liver cells. / Gallai, M.; Kovalszky, I.; Knittel, Thomas; Neubauer, Katrin; Armbrust, Thomas; Ramadori, Giuliano.

In: American Journal of Pathology, Vol. 148, No. 5, 05.1996, p. 1463-1471.

Research output: Contribution to journalArticle

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