Expression of endothelial nitric oxide synthase in the vascular wall during arteriogenesis

Wei Jun Cai, E. Kocsis, Xuegang Luo, Wolfgang Schaper, Jutta Schaper

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Nitric oxide (NO) has been demonstrated to play an important role in angiogenesis, and also to be involved in collateral vessel growth. The expression of endothelial NO synthase (eNOS) is moderated partly by blood flow-induced mechanical factors, i.e., shear stress. The purpose of this study was to evaluate how the expression of eNOS correlates with the development of collateral vessels in dog heart, induced by chronic occlusion of the left circumflex artery. Immunoconfocal microscopy using an antibody against eNOS was used to detect expression of eNOS in different stages of arteriogenesis. Collateral vessels were classified into normal, growing and mature vessels by using the cytoskeleton marker desmin. Expression of the growth factors bFGF and metallproteinase-2 (MMP-2) was also examined. The data show that in normal arteriolar vessels, expression of eNOS is very low, but in growing collateral vessel there is a 6.2-fold increase, which, however, returned to normal levels in mature collateral vessels. The expression of eNOS was localized only in endothelium, either in normal or growing vessels. bFGF was very weakly stained in normal vessels, but highly expressed in growing collateral vessels. MMP-2 was strongly stained in neointima, but very weak in endothelium. In addition, we also examined expression of iNOS because iNOS may be induced in vessel injury or in disease states, but it was not detected in either normal or growing collateral vessels. Our findings indicate that the expression pattern of eNOS is closely associated with the development of collateral vessels, suggesting that eNOS plays an important role in arteriogenesis.

Original languageEnglish
Pages (from-to)193-200
Number of pages8
JournalMolecular and Cellular Biochemistry
Volume264
Issue number1-2
DOIs
Publication statusPublished - Sep 2004

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Nitric Oxide Synthase Type III
Nitric Oxide Synthase
Blood Vessels
Matrix Metalloproteinases
Endothelium
Neointima
Desmin
Cytoskeleton
Shear stress
Microscopy
Intercellular Signaling Peptides and Proteins
Microscopic examination
Nitric Oxide
Blood
Arteries
Dogs
Antibodies
Wounds and Injuries
Growth

Keywords

  • Arteriogenesis
  • Desmin
  • Dog
  • eNOS

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Expression of endothelial nitric oxide synthase in the vascular wall during arteriogenesis. / Cai, Wei Jun; Kocsis, E.; Luo, Xuegang; Schaper, Wolfgang; Schaper, Jutta.

In: Molecular and Cellular Biochemistry, Vol. 264, No. 1-2, 09.2004, p. 193-200.

Research output: Contribution to journalArticle

Cai, Wei Jun ; Kocsis, E. ; Luo, Xuegang ; Schaper, Wolfgang ; Schaper, Jutta. / Expression of endothelial nitric oxide synthase in the vascular wall during arteriogenesis. In: Molecular and Cellular Biochemistry. 2004 ; Vol. 264, No. 1-2. pp. 193-200.
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