We obtained transgenic rice plants carrying the alfalfa (Medicago saliva) Msenod 12 promoters fused to the uidA (GUS) reporter gene. While transient expression of the constructs was not detectable, the Msenod 12B promoter was expressed in the stable rice transformants. GUS activity was detected in proembryogenic calli, whereas in the regenerated plants, without external supply of enod 12 inducers, it was found in the root vascular tissue. This expression pattern was changed, however, in the transgenic rice roots maintained in a 2,4-D-containing medium by microballistic application of the Nod factor, NodRm-IV (C16 : 2,S) from Rhizobium meliloti, the microsymbiont of M. sativa.
|Number of pages||7|
|Publication status||Published - Aug 10 2001|
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