Expression of early nodulin promoter gene in transgenic rice

Rie Terada, S. Ignacimuthu, Petra Bauer, Eva Kondorosi, Michael Schultze, Adam Kondorosi, Ingo Potrykus, Christof Sautter

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Abstract

We obtained transgenic rice plants carrying the alfalfa (Medicago saliva) Msenod 12 promoters fused to the uidA (GUS) reporter gene. While transient expression of the constructs was not detectable, the Msenod 12B promoter was expressed in the stable rice transformants. GUS activity was detected in proembryogenic calli, whereas in the regenerated plants, without external supply of enod 12 inducers, it was found in the root vascular tissue. This expression pattern was changed, however, in the transgenic rice roots maintained in a 2,4-D-containing medium by microballistic application of the Nod factor, NodRm-IV (C16 : 2,S) from Rhizobium meliloti, the microsymbiont of M. sativa.

Original languageEnglish
Pages (from-to)270-276
Number of pages7
JournalCurrent Science
Volume81
Issue number3
Publication statusPublished - Aug 10 2001

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Cite this

Terada, R., Ignacimuthu, S., Bauer, P., Kondorosi, E., Schultze, M., Kondorosi, A., Potrykus, I., & Sautter, C. (2001). Expression of early nodulin promoter gene in transgenic rice. Current Science, 81(3), 270-276.