Expression of differentiated functions in dexamethasone-resistant hepatoma cells

Aniko Venetianer, Zsuzsa Bösze

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The expression of liver-specific functions of different dexamethasone-resistant variants derived from a well-differentiated dexamethasone-sensitive Reuber H35 rat hepatoma cell line (Faza 967) was examined during long-term cultivation. The dexamethasone-sensitive Faza 967 cells are characterized by the activity of tyrosine aminotransferase (TAT) and gluconeogenic enzymes, secretion of serum albumin, and the presence of liver isozymes of alcohol dehydrogenase (L-ADH), aldolase (aldolase-B), and five isoenzymes of lactate dehydrogenase (LDH). The hormone-resistant cells undergo a very dramatic change in expression of most liver-specific functions (dedifferentiation) during long-term culture, in contrast to the sensitive cells in which only certain functions (TAT activity, inducibility, and synthesis of serum albumin) exhibit considerable changes. The hormone-dependent growth sensitivity and the expression of other differentiated functions is not controlled in coordinated way in Faza 967 cells. The time course of the expression of liver-specific functions shows that the cells are resistant before they became ‘dedifferentiated’, i.e., loss of these liver-specific functions is not a prerequisite of the establishment of the hormone-resistant state.

Original languageEnglish
Pages (from-to)70-78
Number of pages9
JournalDifferentiation
Volume25
Issue number1-3
DOIs
Publication statusPublished - Jan 1 1984

    Fingerprint

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

Cite this