Ekspressiia klonirovannogo gena metiltransferazy iz faga SPbetaB Bacillus subtilis.

Translated title of the contribution: Expression of cloned gene for methyltransferase from Bacillus subtilis bacteriophage SPbetaB

F. Baldauf, A. Kiss

Research output: Contribution to journalArticle

Abstract

Expression of the methyltransferase gene from Bacillus subtilis lysogenizing phage SP beta B was studied by analyzing the sensitivity of the hybrid plasmid DNAs to restriction by the enzymes BspRI, Hpall and Mspl. The gene produces the methylase M. BsuP beta BI with specificity for 5'-GGCC. The fragment carrying the SP beta B derived gene also directs the synthesis in E. coli of a second methylase activity (M. BsuP beta BII) with 5'-CCGG specificity. Indirect evidence suggests that the two SP beta B modification activities are encoded by the same gene.

Original languageUndefined/Unknown
Pages (from-to)26-28
Number of pages3
JournalMolekuliarnaia genetika, mikrobiologiia i virusologiia
Issue number2
Publication statusPublished - Feb 1985

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Bacillus Phages
Methyltransferases
Bacillus subtilis
Gene Expression
Genes
DNA Restriction Enzymes
Bacteriophages
Plasmids
Escherichia coli

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Ekspressiia klonirovannogo gena metiltransferazy iz faga SPbetaB Bacillus subtilis. / Baldauf, F.; Kiss, A.

In: Molekuliarnaia genetika, mikrobiologiia i virusologiia, No. 2, 02.1985, p. 26-28.

Research output: Contribution to journalArticle

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