Klaudin-1-expressziós vizsgálatok kutyák daganatos circumanalis mirigyeiben: Immunhisztokémiai tanulmány

Translated title of the contribution: Expression of claudin-1 molecule in canine circumanal gland tumours: Immunohistochemical study

Jakab Csaba, Gálfi Péter, Jerzsele Ákos, L. Balogh, Thuróczy Julianna, Szász Viktor, Sterczer Ágnes

Research output: Contribution to journalArticle

Abstract

The aim of the present study was to characterise the expression pattern of claudin-1 tight junction protein in canine intact circumanal, or hepatoid glands and in pathological proliferative lesions of the hepatoid gland, including nodular hyperplasia, adenoma, epithelioma, differentiated carcinoma and poorly differentiated carcinoma. The present study has evaluated the expression of claudin-1 in 10 intact and 67 hyperplastic and neoplastic lesions of the canine hepatoid gland. The studied lesions included nodular hyperplasias (n=10), adenomas (n=12), epitheliomas (n=15), differentiated carcinomas (n=15) and poorly differentiated carcinomas (n=15). There was diffuse, homogenous, intense expression of claudin-1 in intact canine hepatoid glands, hyperplasias, adenomas and differentiated carcinomas, detected as intense lateral membrane labelling of epithelial cells. The claudin-1 expression was weaker in epitheliomas. The poorly differentiated carcinoma cells never expressed claudin-1. The basal reserve cells of the intact gland, hyperplasia and adenoma showed intense membrane positivity for claudin-1. In epitheliomas the neoplastic basal cells never expressed this tight junction protein. In the epitheliomas some peripheral individual tumour cells showed non-junctional (cytoplasmatic) claudin-1 expressions. Claudin-1 molecule is an integral membrane protein of tight junction structures expressed by canine intact mature circumanal gland cells and peripheral basal reserve cells. Consequently, the authors hypothesize that claudin-1 plays a role in the carcinogenesis of canine circumanal glands. Low claudin-1 expression in epitheliomas and mislocalised claudin-1 expression from the cell membrane to the cytoplasm and the loss of claudin-1 protein in poorly differentiated carcinomas may be the molecular phenomenon of increasing cellular disorientation, motility, invasion, and metastasis of tumour cells.

Original languageHungarian
Pages (from-to)591-600
Number of pages10
JournalMagyar Allatorvosok Lapja
Volume132
Issue number10
Publication statusPublished - Oct 2010

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Perianal Glands
Claudin-1
carcinoma
Canidae
Carcinoma
adenoma
neoplasms
dogs
hyperplasia
tight junctions
Neoplasms
lesions (animal)
Adenoma
Hyperplasia
cells
Tight Junction Proteins
proteins
cell movement
membrane proteins
metastasis

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Csaba, J., Péter, G., Ákos, J., Balogh, L., Julianna, T., Viktor, S., & Ágnes, S. (2010). Klaudin-1-expressziós vizsgálatok kutyák daganatos circumanalis mirigyeiben: Immunhisztokémiai tanulmány. Magyar Allatorvosok Lapja, 132(10), 591-600.

Klaudin-1-expressziós vizsgálatok kutyák daganatos circumanalis mirigyeiben : Immunhisztokémiai tanulmány. / Csaba, Jakab; Péter, Gálfi; Ákos, Jerzsele; Balogh, L.; Julianna, Thuróczy; Viktor, Szász; Ágnes, Sterczer.

In: Magyar Allatorvosok Lapja, Vol. 132, No. 10, 10.2010, p. 591-600.

Research output: Contribution to journalArticle

Csaba, J, Péter, G, Ákos, J, Balogh, L, Julianna, T, Viktor, S & Ágnes, S 2010, 'Klaudin-1-expressziós vizsgálatok kutyák daganatos circumanalis mirigyeiben: Immunhisztokémiai tanulmány', Magyar Allatorvosok Lapja, vol. 132, no. 10, pp. 591-600.
Csaba, Jakab ; Péter, Gálfi ; Ákos, Jerzsele ; Balogh, L. ; Julianna, Thuróczy ; Viktor, Szász ; Ágnes, Sterczer. / Klaudin-1-expressziós vizsgálatok kutyák daganatos circumanalis mirigyeiben : Immunhisztokémiai tanulmány. In: Magyar Allatorvosok Lapja. 2010 ; Vol. 132, No. 10. pp. 591-600.
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abstract = "The aim of the present study was to characterise the expression pattern of claudin-1 tight junction protein in canine intact circumanal, or hepatoid glands and in pathological proliferative lesions of the hepatoid gland, including nodular hyperplasia, adenoma, epithelioma, differentiated carcinoma and poorly differentiated carcinoma. The present study has evaluated the expression of claudin-1 in 10 intact and 67 hyperplastic and neoplastic lesions of the canine hepatoid gland. The studied lesions included nodular hyperplasias (n=10), adenomas (n=12), epitheliomas (n=15), differentiated carcinomas (n=15) and poorly differentiated carcinomas (n=15). There was diffuse, homogenous, intense expression of claudin-1 in intact canine hepatoid glands, hyperplasias, adenomas and differentiated carcinomas, detected as intense lateral membrane labelling of epithelial cells. The claudin-1 expression was weaker in epitheliomas. The poorly differentiated carcinoma cells never expressed claudin-1. The basal reserve cells of the intact gland, hyperplasia and adenoma showed intense membrane positivity for claudin-1. In epitheliomas the neoplastic basal cells never expressed this tight junction protein. In the epitheliomas some peripheral individual tumour cells showed non-junctional (cytoplasmatic) claudin-1 expressions. Claudin-1 molecule is an integral membrane protein of tight junction structures expressed by canine intact mature circumanal gland cells and peripheral basal reserve cells. Consequently, the authors hypothesize that claudin-1 plays a role in the carcinogenesis of canine circumanal glands. Low claudin-1 expression in epitheliomas and mislocalised claudin-1 expression from the cell membrane to the cytoplasm and the loss of claudin-1 protein in poorly differentiated carcinomas may be the molecular phenomenon of increasing cellular disorientation, motility, invasion, and metastasis of tumour cells.",
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