Expression of claudin-1 in canine peripheral nerve sheath tumours and perivascular wall tumours. Immunohistochemical study

C. S. Jakab, P. Gálfi, A. Jerzsele, Z. Szabó, T. Németh, A. Sterczer, M. Rusvai, L. Ózsvári

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Aims: A peripheral nerve sheath tumour consists of neoplastic Schwann cells or perineurial cells, or a mixture of Schwann cells, perineurial cells and fibroblasts. The first aim of the present study was to characterise the expression of the claudin-1 tight junction protein in canine intact peripheral nerves, canine benign peripheral nerve sheath tumours (cBPNSTs), such as schwannomas, neurofibromas, perineuriomas and canine malignant peripheral nerve sheath tumours (cMPNSTs), and in different other benign and malignant canine spindle cell tumours. The second aim of the present study was to examine whether claudin-1 can help to distinguish the subgroups of canine perivascular wall tumours. Methods and results: The biopsy and necropsy samples (n=203) included 10 intact peripheral nerves, 20 cBPNSTs (4 schwannomas, 8 neurofibromas, 8 perineuriomas), 16 cMPNSTs, 6 psammomatous meningiomas, 6 dermatofibromas, 6 leiomyomas, 6 myxomas, 4 spindle cell hemangiomas, 2 spindle cell lipomas, 6 fibrohistiocytic nodules, 8 fibrosarcomas, 8 leiomyosarcomas, 6 myxosarcomas, 8 hemangiosarcomas, 8 anaplastic sarcomas, 8 amelanotic spindle cell melanomas, 8 histiocytic sarcomas, 8 spindle cell carcinomas, 8 myoepitheliomas, 8 complex carcinomas, 5 cardiac rhabdomyosarcomas, 4 synovial sarcomas, 5 osteosarcomas, 4 chondrosarcomas and 4 liposarcomas; 31 canine perivascular wall tumours: 10 hemangiopericytomas, 8 myopericytomas, 6 angio leiomyomas, 4 angioleiomyosarcomas, 3 angiofibromas The immunohistochemical panel consisted of humanized antibodies: anti-claudin-1, anti-neuron specific enolase anti-S-100 protein, anti-α-smooth muscle actin, anti vimentin, anti-cytokeratin AE1-AE3, anti-claudin-5 anti-Melan-A and anti-heavy caldesmon, anti-calponin and anti-desmin. The intact perineurial cells, al perineuriomas, neurofibromas, cMPNSTs, spindle cel carcinomas and epithelial components of the complex carcinomas, all hemangiopericytomas and myo pericytomas showed claudin-1 positivity. The schwannomas and other spindle shape cell tumours were negative for claudin-1. Conclusion: Our findings suggest that an antibody against claudin-1, in combination with other antibodies can be used as a novel diagnostic tool to differentiate canine peripheral nerve sheath tumours from othe fusocellular tumours, and anti-claudin-1, together with other antibodies, can also be used to subclassify cBPNSTs. Furthermore, analysis of claudin-1 expression can help to differentiate between subgroups of canine perivascular wall tumours.

Original languageEnglish
Pages (from-to)905-917
Number of pages13
JournalHistology and Histopathology
Volume27
Issue number7
Publication statusPublished - Jul 2012

Fingerprint

Claudin-1
Nerve Sheath Neoplasms
Canidae
Neurilemmoma
Neoplasms
Neurofibroma
Hemangiopericytoma
Schwann Cells
Leiomyoma
Carcinoma
Peripheral Nerves
Myxosarcoma
Antibodies
Claudin-5
Myoepithelioma
MART-1 Antigen
Histiocytic Sarcoma
Angiofibroma
Benign Fibrous Histiocytoma
Calmodulin-Binding Proteins

Keywords

  • Canine peripheral nerve sheath tumour
  • Canine perivascular wall tumours
  • Canine spindle cell tumours
  • Claudin-1
  • Differential diagnosis
  • Hemangiopericytoma
  • Immuno-histochemistry
  • Immunohistochemical panel
  • Neurofibroma
  • Perineurioma
  • Schwannoma

ASJC Scopus subject areas

  • Histology
  • Pathology and Forensic Medicine

Cite this

Jakab, C. S., Gálfi, P., Jerzsele, A., Szabó, Z., Németh, T., Sterczer, A., ... Ózsvári, L. (2012). Expression of claudin-1 in canine peripheral nerve sheath tumours and perivascular wall tumours. Immunohistochemical study. Histology and Histopathology, 27(7), 905-917.

Expression of claudin-1 in canine peripheral nerve sheath tumours and perivascular wall tumours. Immunohistochemical study. / Jakab, C. S.; Gálfi, P.; Jerzsele, A.; Szabó, Z.; Németh, T.; Sterczer, A.; Rusvai, M.; Ózsvári, L.

In: Histology and Histopathology, Vol. 27, No. 7, 07.2012, p. 905-917.

Research output: Contribution to journalArticle

Jakab, CS, Gálfi, P, Jerzsele, A, Szabó, Z, Németh, T, Sterczer, A, Rusvai, M & Ózsvári, L 2012, 'Expression of claudin-1 in canine peripheral nerve sheath tumours and perivascular wall tumours. Immunohistochemical study', Histology and Histopathology, vol. 27, no. 7, pp. 905-917.
Jakab, C. S. ; Gálfi, P. ; Jerzsele, A. ; Szabó, Z. ; Németh, T. ; Sterczer, A. ; Rusvai, M. ; Ózsvári, L. / Expression of claudin-1 in canine peripheral nerve sheath tumours and perivascular wall tumours. Immunohistochemical study. In: Histology and Histopathology. 2012 ; Vol. 27, No. 7. pp. 905-917.
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T1 - Expression of claudin-1 in canine peripheral nerve sheath tumours and perivascular wall tumours. Immunohistochemical study

AU - Jakab, C. S.

AU - Gálfi, P.

AU - Jerzsele, A.

AU - Szabó, Z.

AU - Németh, T.

AU - Sterczer, A.

AU - Rusvai, M.

AU - Ózsvári, L.

PY - 2012/7

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N2 - Aims: A peripheral nerve sheath tumour consists of neoplastic Schwann cells or perineurial cells, or a mixture of Schwann cells, perineurial cells and fibroblasts. The first aim of the present study was to characterise the expression of the claudin-1 tight junction protein in canine intact peripheral nerves, canine benign peripheral nerve sheath tumours (cBPNSTs), such as schwannomas, neurofibromas, perineuriomas and canine malignant peripheral nerve sheath tumours (cMPNSTs), and in different other benign and malignant canine spindle cell tumours. The second aim of the present study was to examine whether claudin-1 can help to distinguish the subgroups of canine perivascular wall tumours. Methods and results: The biopsy and necropsy samples (n=203) included 10 intact peripheral nerves, 20 cBPNSTs (4 schwannomas, 8 neurofibromas, 8 perineuriomas), 16 cMPNSTs, 6 psammomatous meningiomas, 6 dermatofibromas, 6 leiomyomas, 6 myxomas, 4 spindle cell hemangiomas, 2 spindle cell lipomas, 6 fibrohistiocytic nodules, 8 fibrosarcomas, 8 leiomyosarcomas, 6 myxosarcomas, 8 hemangiosarcomas, 8 anaplastic sarcomas, 8 amelanotic spindle cell melanomas, 8 histiocytic sarcomas, 8 spindle cell carcinomas, 8 myoepitheliomas, 8 complex carcinomas, 5 cardiac rhabdomyosarcomas, 4 synovial sarcomas, 5 osteosarcomas, 4 chondrosarcomas and 4 liposarcomas; 31 canine perivascular wall tumours: 10 hemangiopericytomas, 8 myopericytomas, 6 angio leiomyomas, 4 angioleiomyosarcomas, 3 angiofibromas The immunohistochemical panel consisted of humanized antibodies: anti-claudin-1, anti-neuron specific enolase anti-S-100 protein, anti-α-smooth muscle actin, anti vimentin, anti-cytokeratin AE1-AE3, anti-claudin-5 anti-Melan-A and anti-heavy caldesmon, anti-calponin and anti-desmin. The intact perineurial cells, al perineuriomas, neurofibromas, cMPNSTs, spindle cel carcinomas and epithelial components of the complex carcinomas, all hemangiopericytomas and myo pericytomas showed claudin-1 positivity. The schwannomas and other spindle shape cell tumours were negative for claudin-1. Conclusion: Our findings suggest that an antibody against claudin-1, in combination with other antibodies can be used as a novel diagnostic tool to differentiate canine peripheral nerve sheath tumours from othe fusocellular tumours, and anti-claudin-1, together with other antibodies, can also be used to subclassify cBPNSTs. Furthermore, analysis of claudin-1 expression can help to differentiate between subgroups of canine perivascular wall tumours.

AB - Aims: A peripheral nerve sheath tumour consists of neoplastic Schwann cells or perineurial cells, or a mixture of Schwann cells, perineurial cells and fibroblasts. The first aim of the present study was to characterise the expression of the claudin-1 tight junction protein in canine intact peripheral nerves, canine benign peripheral nerve sheath tumours (cBPNSTs), such as schwannomas, neurofibromas, perineuriomas and canine malignant peripheral nerve sheath tumours (cMPNSTs), and in different other benign and malignant canine spindle cell tumours. The second aim of the present study was to examine whether claudin-1 can help to distinguish the subgroups of canine perivascular wall tumours. Methods and results: The biopsy and necropsy samples (n=203) included 10 intact peripheral nerves, 20 cBPNSTs (4 schwannomas, 8 neurofibromas, 8 perineuriomas), 16 cMPNSTs, 6 psammomatous meningiomas, 6 dermatofibromas, 6 leiomyomas, 6 myxomas, 4 spindle cell hemangiomas, 2 spindle cell lipomas, 6 fibrohistiocytic nodules, 8 fibrosarcomas, 8 leiomyosarcomas, 6 myxosarcomas, 8 hemangiosarcomas, 8 anaplastic sarcomas, 8 amelanotic spindle cell melanomas, 8 histiocytic sarcomas, 8 spindle cell carcinomas, 8 myoepitheliomas, 8 complex carcinomas, 5 cardiac rhabdomyosarcomas, 4 synovial sarcomas, 5 osteosarcomas, 4 chondrosarcomas and 4 liposarcomas; 31 canine perivascular wall tumours: 10 hemangiopericytomas, 8 myopericytomas, 6 angio leiomyomas, 4 angioleiomyosarcomas, 3 angiofibromas The immunohistochemical panel consisted of humanized antibodies: anti-claudin-1, anti-neuron specific enolase anti-S-100 protein, anti-α-smooth muscle actin, anti vimentin, anti-cytokeratin AE1-AE3, anti-claudin-5 anti-Melan-A and anti-heavy caldesmon, anti-calponin and anti-desmin. The intact perineurial cells, al perineuriomas, neurofibromas, cMPNSTs, spindle cel carcinomas and epithelial components of the complex carcinomas, all hemangiopericytomas and myo pericytomas showed claudin-1 positivity. The schwannomas and other spindle shape cell tumours were negative for claudin-1. Conclusion: Our findings suggest that an antibody against claudin-1, in combination with other antibodies can be used as a novel diagnostic tool to differentiate canine peripheral nerve sheath tumours from othe fusocellular tumours, and anti-claudin-1, together with other antibodies, can also be used to subclassify cBPNSTs. Furthermore, analysis of claudin-1 expression can help to differentiate between subgroups of canine perivascular wall tumours.

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KW - Differential diagnosis

KW - Hemangiopericytoma

KW - Immuno-histochemistry

KW - Immunohistochemical panel

KW - Neurofibroma

KW - Perineurioma

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