Expression of cholera toxin B subunit in transgenic rice endosperm

Maria Oszvald, Tae Jin Kang, Sandor Tomoskozi, Barnabas Jenes, Tae Geum Kim, Youn Soo Cha, Laszlo Tamas, Moon Sik Yang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The synthetic cholera toxin B subunit (CTB) gene, modified according to the optimized codon usage of plant genes, was introduced into a plant expression vector and expressed under the control of the Bx17 HMW (high molecular weight) wheat endosperm-specific promoter containing an intron of the rice act1. The recombinant vector was transformed into rice plants using a biolistic-mediated transformation method. Stable integration of the synthetic CTB gene into the chromosomal DNA was confirmed by PCR amplification analysis. A high level of CTB (2.1% of total soluble protein) was expressed in the endosperm tissue of the transgenic rice plants. The synthetic CTB produced only in the rice endosperm demonstrated strong affinity for GM1-ganglioside, thereby suggesting that the CTB subunits formed an active pentamer. The successful expression of CTB genes in transgenic plants makes it a powerful tool for the development of a plant-derived edible vaccine.

Original languageEnglish
Pages (from-to)261-268
Number of pages8
JournalMolecular Biotechnology
Volume40
Issue number3
DOIs
Publication statusPublished - Nov 2008

Fingerprint

Endosperm
Cholera Toxin
Genes
Genetically Modified Plants
Vaccines
Edible Vaccines
Biolistics
G(M1) Ganglioside
Amplification
Plant Genes
DNA
Plant Development
Molecular weight
Tissue
Proteins
Codon
Introns
Triticum
Oryza
Molecular Weight

Keywords

  • Cholera toxin B subunit
  • Endosperm-specific promoter
  • Plant-based vaccine
  • Rice

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Molecular Biology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Oszvald, M., Kang, T. J., Tomoskozi, S., Jenes, B., Kim, T. G., Cha, Y. S., ... Yang, M. S. (2008). Expression of cholera toxin B subunit in transgenic rice endosperm. Molecular Biotechnology, 40(3), 261-268. https://doi.org/10.1007/s12033-008-9083-2

Expression of cholera toxin B subunit in transgenic rice endosperm. / Oszvald, Maria; Kang, Tae Jin; Tomoskozi, Sandor; Jenes, Barnabas; Kim, Tae Geum; Cha, Youn Soo; Tamas, Laszlo; Yang, Moon Sik.

In: Molecular Biotechnology, Vol. 40, No. 3, 11.2008, p. 261-268.

Research output: Contribution to journalArticle

Oszvald, M, Kang, TJ, Tomoskozi, S, Jenes, B, Kim, TG, Cha, YS, Tamas, L & Yang, MS 2008, 'Expression of cholera toxin B subunit in transgenic rice endosperm', Molecular Biotechnology, vol. 40, no. 3, pp. 261-268. https://doi.org/10.1007/s12033-008-9083-2
Oszvald M, Kang TJ, Tomoskozi S, Jenes B, Kim TG, Cha YS et al. Expression of cholera toxin B subunit in transgenic rice endosperm. Molecular Biotechnology. 2008 Nov;40(3):261-268. https://doi.org/10.1007/s12033-008-9083-2
Oszvald, Maria ; Kang, Tae Jin ; Tomoskozi, Sandor ; Jenes, Barnabas ; Kim, Tae Geum ; Cha, Youn Soo ; Tamas, Laszlo ; Yang, Moon Sik. / Expression of cholera toxin B subunit in transgenic rice endosperm. In: Molecular Biotechnology. 2008 ; Vol. 40, No. 3. pp. 261-268.
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