Expression of Certain Leukemia/Lymphoma Related microRNAs and its Correlation with Prognosis in Childhood Acute Lymphoblastic Leukemia

Karolina Nemes, Monika Csóka, Noémi Nagy, Ágnes Márk, Zsófia Váradi, Titanilla Dankó, Gábor Kovács, L. Kópper, A. Sebestyén

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

In spite of the improved efficacy of therapy, it still fails in 15–20 % of childhood acute lymphoblastic leukemia (ALL) patients. Recently, altered expression of certain miRNAs (miRs) have been described in ALL with potential effect on prognosis. Presence of certain miRs (miRNA-16, −21, −24, −29b, −128b, −142-3p, −155, −223) was characterized in human lymphoma and leukemia cells by real-time PCR. Expression of miRs in pediatric ALL patients (n = 24) was measured before chemotherapy, at conventional response checkpoints and at relapse. Correlation between altered miR expression and response to prednisolone at day 8 of therapy and long term prognosis was statistically analysed. Overexpression of “oncomiR/inflammamiR”-21 – which is characteristic in different tumors—was missing in human ALL cells. However, higher expression of miR-128b and lower expression of miR-223 is generally characteristic for human ALL cell lines and ALL cells isolated from pediatric patients. Correlation was shown between miR-128b expression and prognosis, prednisolone response and survival data in childhood ALL. Expression of miR-128b and miR-223—both are leukemia specific—changed in parallel with percentage of bone marrow blasts in remission and during relapse. Therefore, we suggest that overexpression of miR-128b and downregulation of miR-223 shows a significant correlation with treatment response and prognosis in childhood ALL.

Original languageEnglish
Pages (from-to)597-604
Number of pages8
JournalPathology and Oncology Research
Volume21
Issue number3
DOIs
Publication statusPublished - Nov 12 2014

Fingerprint

MicroRNAs
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Lymphoma
Leukemia
Prednisolone
Pediatrics
Recurrence
Real-Time Polymerase Chain Reaction
Therapeutics
Down-Regulation
Bone Marrow
Drug Therapy
Cell Line
Survival

Keywords

  • Childhood ALL
  • miRNA-128b miRNA-223
  • Prognosis
  • Relapse

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pathology and Forensic Medicine

Cite this

Expression of Certain Leukemia/Lymphoma Related microRNAs and its Correlation with Prognosis in Childhood Acute Lymphoblastic Leukemia. / Nemes, Karolina; Csóka, Monika; Nagy, Noémi; Márk, Ágnes; Váradi, Zsófia; Dankó, Titanilla; Kovács, Gábor; Kópper, L.; Sebestyén, A.

In: Pathology and Oncology Research, Vol. 21, No. 3, 12.11.2014, p. 597-604.

Research output: Contribution to journalArticle

Nemes, Karolina ; Csóka, Monika ; Nagy, Noémi ; Márk, Ágnes ; Váradi, Zsófia ; Dankó, Titanilla ; Kovács, Gábor ; Kópper, L. ; Sebestyén, A. / Expression of Certain Leukemia/Lymphoma Related microRNAs and its Correlation with Prognosis in Childhood Acute Lymphoblastic Leukemia. In: Pathology and Oncology Research. 2014 ; Vol. 21, No. 3. pp. 597-604.
@article{536c857de17d4217b158c5f2bc1f5ff6,
title = "Expression of Certain Leukemia/Lymphoma Related microRNAs and its Correlation with Prognosis in Childhood Acute Lymphoblastic Leukemia",
abstract = "In spite of the improved efficacy of therapy, it still fails in 15–20 {\%} of childhood acute lymphoblastic leukemia (ALL) patients. Recently, altered expression of certain miRNAs (miRs) have been described in ALL with potential effect on prognosis. Presence of certain miRs (miRNA-16, −21, −24, −29b, −128b, −142-3p, −155, −223) was characterized in human lymphoma and leukemia cells by real-time PCR. Expression of miRs in pediatric ALL patients (n = 24) was measured before chemotherapy, at conventional response checkpoints and at relapse. Correlation between altered miR expression and response to prednisolone at day 8 of therapy and long term prognosis was statistically analysed. Overexpression of “oncomiR/inflammamiR”-21 – which is characteristic in different tumors—was missing in human ALL cells. However, higher expression of miR-128b and lower expression of miR-223 is generally characteristic for human ALL cell lines and ALL cells isolated from pediatric patients. Correlation was shown between miR-128b expression and prognosis, prednisolone response and survival data in childhood ALL. Expression of miR-128b and miR-223—both are leukemia specific—changed in parallel with percentage of bone marrow blasts in remission and during relapse. Therefore, we suggest that overexpression of miR-128b and downregulation of miR-223 shows a significant correlation with treatment response and prognosis in childhood ALL.",
keywords = "Childhood ALL, miRNA-128b miRNA-223, Prognosis, Relapse",
author = "Karolina Nemes and Monika Cs{\'o}ka and No{\'e}mi Nagy and {\'A}gnes M{\'a}rk and Zs{\'o}fia V{\'a}radi and Titanilla Dank{\'o} and G{\'a}bor Kov{\'a}cs and L. K{\'o}pper and A. Sebesty{\'e}n",
year = "2014",
month = "11",
day = "12",
doi = "10.1007/s12253-014-9861-z",
language = "English",
volume = "21",
pages = "597--604",
journal = "Pathology and Oncology Research",
issn = "1219-4956",
publisher = "Springer Netherlands",
number = "3",

}

TY - JOUR

T1 - Expression of Certain Leukemia/Lymphoma Related microRNAs and its Correlation with Prognosis in Childhood Acute Lymphoblastic Leukemia

AU - Nemes, Karolina

AU - Csóka, Monika

AU - Nagy, Noémi

AU - Márk, Ágnes

AU - Váradi, Zsófia

AU - Dankó, Titanilla

AU - Kovács, Gábor

AU - Kópper, L.

AU - Sebestyén, A.

PY - 2014/11/12

Y1 - 2014/11/12

N2 - In spite of the improved efficacy of therapy, it still fails in 15–20 % of childhood acute lymphoblastic leukemia (ALL) patients. Recently, altered expression of certain miRNAs (miRs) have been described in ALL with potential effect on prognosis. Presence of certain miRs (miRNA-16, −21, −24, −29b, −128b, −142-3p, −155, −223) was characterized in human lymphoma and leukemia cells by real-time PCR. Expression of miRs in pediatric ALL patients (n = 24) was measured before chemotherapy, at conventional response checkpoints and at relapse. Correlation between altered miR expression and response to prednisolone at day 8 of therapy and long term prognosis was statistically analysed. Overexpression of “oncomiR/inflammamiR”-21 – which is characteristic in different tumors—was missing in human ALL cells. However, higher expression of miR-128b and lower expression of miR-223 is generally characteristic for human ALL cell lines and ALL cells isolated from pediatric patients. Correlation was shown between miR-128b expression and prognosis, prednisolone response and survival data in childhood ALL. Expression of miR-128b and miR-223—both are leukemia specific—changed in parallel with percentage of bone marrow blasts in remission and during relapse. Therefore, we suggest that overexpression of miR-128b and downregulation of miR-223 shows a significant correlation with treatment response and prognosis in childhood ALL.

AB - In spite of the improved efficacy of therapy, it still fails in 15–20 % of childhood acute lymphoblastic leukemia (ALL) patients. Recently, altered expression of certain miRNAs (miRs) have been described in ALL with potential effect on prognosis. Presence of certain miRs (miRNA-16, −21, −24, −29b, −128b, −142-3p, −155, −223) was characterized in human lymphoma and leukemia cells by real-time PCR. Expression of miRs in pediatric ALL patients (n = 24) was measured before chemotherapy, at conventional response checkpoints and at relapse. Correlation between altered miR expression and response to prednisolone at day 8 of therapy and long term prognosis was statistically analysed. Overexpression of “oncomiR/inflammamiR”-21 – which is characteristic in different tumors—was missing in human ALL cells. However, higher expression of miR-128b and lower expression of miR-223 is generally characteristic for human ALL cell lines and ALL cells isolated from pediatric patients. Correlation was shown between miR-128b expression and prognosis, prednisolone response and survival data in childhood ALL. Expression of miR-128b and miR-223—both are leukemia specific—changed in parallel with percentage of bone marrow blasts in remission and during relapse. Therefore, we suggest that overexpression of miR-128b and downregulation of miR-223 shows a significant correlation with treatment response and prognosis in childhood ALL.

KW - Childhood ALL

KW - miRNA-128b miRNA-223

KW - Prognosis

KW - Relapse

UR - http://www.scopus.com/inward/record.url?scp=84939416369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84939416369&partnerID=8YFLogxK

U2 - 10.1007/s12253-014-9861-z

DO - 10.1007/s12253-014-9861-z

M3 - Article

C2 - 25388103

AN - SCOPUS:84939416369

VL - 21

SP - 597

EP - 604

JO - Pathology and Oncology Research

JF - Pathology and Oncology Research

SN - 1219-4956

IS - 3

ER -