Expression of CD44v3 protein in human endothelial cells in vitro and in tumoral microvessels in vivo

C. Forster-Horváth, L. Mészáros, E. Rásó, B. Döme, A. Ladányi, M. Morini, A. Albini, J. Tímár

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The most universal angiogenic cytokines (VEGF, bFGF, HGF) are all heparin-binding proteins, the function of which is dependent on cell surface heparan sulfate proteoglycans (HSPG). Several proteoglycans have been demonstrated in endothelial cells, but only glypican-1 from the cell surface HSPG subfamily was documented at protein level. Here, we show that CD44v3 is expressed in human immortalized endothelial cells [anchorage-dependent human umbilical vein endothelial cells (HUVEC) and anchorage-independent Kaposi sarcoma (KS-Imm)] at mRNA and protein level, but is absent from the primary culture of human brain microvascular endothelial cells. We have shown that CD44v3 has a large cytoplasmic pool in endothelial cells, but a limited surface expression, mainly at filopodia, colocalized with MMP-2. Angiogenic factors like VEGF or bFGF did not affect surface detection of CD44v3 suggesting a constitutive expression. The putative functional role for endothelial cell surface CD44v3 was identified in chemotaxis assay when anti-CD44v3 antibody pretreatment proved to be inhibitory for HUVEC. Furthermore, we provided evidence for the CD44v3 protein expression in human endothelial cells in vivo in peritumoral microvessels of both human melanoma and glottic cancers, suggesting a role for this part-time heparan sulfate proteoglycan in tumor induced angiogenesis.

Original languageEnglish
Pages (from-to)110-118
Number of pages9
JournalMicrovascular Research
Volume68
Issue number2
DOIs
Publication statusPublished - Sep 2004

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Endothelial cells
Microvessels
Endothelial Cells
Heparan Sulfate Proteoglycans
Proteins
Human Umbilical Vein Endothelial Cells
Vascular Endothelial Growth Factor A
Glypicans
Tongue Neoplasms
Pseudopodia
Angiogenesis Inducing Agents
Kaposi's Sarcoma
Chemotaxis
Proteoglycans
Matrix Metalloproteinases
In Vitro Techniques
Anti-Idiotypic Antibodies
Melanoma
Cell culture
Cytokines

Keywords

  • CD44v3
  • Microvessels
  • Proteoglycan

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine

Cite this

Expression of CD44v3 protein in human endothelial cells in vitro and in tumoral microvessels in vivo. / Forster-Horváth, C.; Mészáros, L.; Rásó, E.; Döme, B.; Ladányi, A.; Morini, M.; Albini, A.; Tímár, J.

In: Microvascular Research, Vol. 68, No. 2, 09.2004, p. 110-118.

Research output: Contribution to journalArticle

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