Expression of bone matrix proteins during de novo bone formation using a bovine collagen and platelet-rich plasma (prp) - An immunohistochemical analysis

M. Thorwarth, S. Rupprecht, S. Falk, E. Felszeghy, J. Wiltfang, K. A. Schlegel

Research output: Contribution to journalArticle

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Abstract

This animal study (domestic pig) examined the bone formation after filling defined defects with autogenous bone or a collagen lyophilisat in combination with Platelet-rich-plasma (PRP) by evaluating bone matrix proteins. Six groups, both materials with and without PRP in two concentrations (+1, +2) were compared to untreated bone by means of immunohistochemistry at 2, 4, 12 and 26 weeks. BMP-2 expression was increased at 2 weeks in the collagen+1 group and after 4 weeks in the collagen+1 and +2 group. Collagen-I expression was increased at 2 weeks in all collagen groups. After 4 weeks raised levels were observed after adding the higher concentrated PRP to bone and the collagen material. Osteocalcin expression was enhanced at 2 weeks in all collagen groups and the autogenous bone+PRP1 group, after 4 weeks in the bone and collagen +2 groups. At 12 weeks higher values were observed after adding higher concentrated PRP to bone. Osteonectin and especially osteopontin were confirmed to be effective markers of early bone formation in all specimens. The described setting allows to combine established techniques (microradiography, light microscopy) with approaches to explore the underlying biology (immunohistochemistry) on the same specimen.

Original languageEnglish
Pages (from-to)2575-2584
Number of pages10
JournalBiomaterials
Volume26
Issue number15
DOIs
Publication statusPublished - May 2005

Fingerprint

Bone Matrix
Platelet-Rich Plasma
Platelets
Collagen
Osteogenesis
Bone
Proteins
Plasmas
Bone and Bones
Immunohistochemistry
Osteonectin
Microradiography
Sus scrofa
Osteopontin
Osteocalcin
Microscopy
Optical microscopy
Animals
Light
Defects

Keywords

  • Bone matrix proteins
  • Bone regeneration
  • Bone substitutes
  • Bovine collagen
  • Platelet-rich plasma
  • Prospective study

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Expression of bone matrix proteins during de novo bone formation using a bovine collagen and platelet-rich plasma (prp) - An immunohistochemical analysis. / Thorwarth, M.; Rupprecht, S.; Falk, S.; Felszeghy, E.; Wiltfang, J.; Schlegel, K. A.

In: Biomaterials, Vol. 26, No. 15, 05.2005, p. 2575-2584.

Research output: Contribution to journalArticle

Thorwarth, M. ; Rupprecht, S. ; Falk, S. ; Felszeghy, E. ; Wiltfang, J. ; Schlegel, K. A. / Expression of bone matrix proteins during de novo bone formation using a bovine collagen and platelet-rich plasma (prp) - An immunohistochemical analysis. In: Biomaterials. 2005 ; Vol. 26, No. 15. pp. 2575-2584.
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